PEG NHS ester linkers are commonly used for crosslinking and labeling peptides and proteins. They react quickly with primary amine groups at the N-terminus and lysine residues of polypeptides. These amines, located on the surface of proteins, are easily accessible for bioconjugation. Their nucleophilic nature makes them ideal for reacting with PEG-NHS esters in protein labeling and crosslinking.
Moreover, the functional groups at the opposite end of the linkers allow further conjugation with biomolecules or drug targets. Adding a PEG spacer improves the linker’s solubility, making it perfect for antibody-drug conjugations (ADC) in aqueous environments.
Key Features:
Quick Reaction: PEG NHS esters react efficiently with primary amines on protein surfaces.
Dual Functionality: The opposite end allows further biomolecule or drug conjugation.
Improved Solubility: The PEG spacer enhances solubility, ideal for ADCs in water-based processes.
Applications:
Fast protein and peptide labeling.
Efficient crosslinking for drug delivery.
Ideal for antibody-drug conjugations in aqueous media.
PEG NHS ester linkers provide versatility and efficiency, making them essential for protein labeling and conjugation.