Sulfo Cyanine5 (Cy5) dye is a fluorescent molecule that is widely used in various applications such as molecular biology, biochemistry, and biomedical research. Cy5 dye is a cyanine-based dye that emits red fluorescence when excited by light of appropriate wavelengths. The sulfo group attached to the dye molecule improves the solubility of the dye in aqueous solutions, making it suitable for labeling biomolecules such as proteins, nucleic acids, and carbohydrates.
Cy5 dye is commonly used in fluorescence microscopy, flow cytometry, and DNA microarray analysis. In fluorescence microscopy, Cy5-labeled biomolecules can be visualized in living cells or tissues with high sensitivity and specificity. In flow cytometry, Cy5 dye is often used in multicolor experiments for the detection and quantification of specific cell types or biomolecules. In DNA microarray analysis, Cy5 dye is frequently used as a labeling agent to detect gene expression levels.