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Sulfo-Cyanine3.5 (Sulfo-Cy3.5) is a bright, water-soluble fluorescent dye ideal for biological applications. Known for its high quantum yield, photostability, and broad absorption, it excels in fluorescence microscopy, flow cytometry, and live cell imaging. Sulfo-Cy3.5 is perfect for labeling proteins, nucleic acids, and antibodies due to its high fluorescence intensity and low molecular weight.

Sulfo-Cyanine3.5

Cat# Name Structure M.W. Purity Pricing
AP12770Sulfo-Cyanine3.5 carboxylic acid991.26≥ (95%) Pricing
AP12768Sulfo-Cyanine3.5 alkyne1028.32≥ (95%) Pricing
AP13142sulfo-Cyanine3.5 amine1051.38≥95% Pricing
AP12769Sulfo-Cyanine3.5 azide1073.37≥ (95%) Pricing
AP12771Sulfo-Cyanine3.5 NHS ester1088.33≥ (95%) Pricing

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    Sulfo-Cyanine3.5 Dye: Spectral Properties and Applications

    Sulfo-Cyanine3.5 (Sulfo-Cy3.5) is a water-soluble, orange-red fluorescent dye known for its excellent photostability, high quantum yield, and strong fluorescence in the visible spectrum. It is commonly used in bioconjugation, labeling of proteins and nucleic acids, and advanced imaging techniques, offering low background and bright signals in aqueous environments.

    Spectral Properties of Sulfo-Cyanine3.5 Dye:

    • Absorption Maximum: ~581 nm
    • Emission Maximum: ~596 nm
    • Stokes Shift: ~15 nm
    • High Quantum Yield: Provides bright and efficient fluorescence.
    • Enhanced Solubility: Sulfonate groups improve water solubility, making it ideal for biological assays.

    Applications of Sulfo-Cyanine3.5 Dye:

    • Fluorescence Microscopy: Provides clear, high-resolution imaging of cells and tissues.
    • Flow Cytometry: Offers distinct signals for precise cell sorting and analysis.
    • Bioconjugation: Efficiently labels proteins, nucleic acids, and other biomolecules.
    • Molecular Probes: Effective in bioanalytical assays to study molecular interactions.

    Sulfo-Cy3.5’s bright fluorescence, excellent stability, and enhanced solubility make it a versatile tool for researchers needing reliable fluorescent labeling in various biological and biomedical applications.

     

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