Sulfo-Cyanine3.5

Sulfo-Cyanine3.5 Dye: Spectral Properties and Applications

Sulfo-Cyanine3.5 (Sulfo-Cy3.5) is a water-soluble, orange-red fluorescent dye known for its excellent photostability, high quantum yield, and strong fluorescence in the visible spectrum. It is commonly used in bioconjugation, labeling of proteins and nucleic acids, and advanced imaging techniques, offering low background and bright signals in aqueous environments.

Spectral Properties of Sulfo-Cyanine3.5 Dye:

• Absorption Maximum: ~581 nm
• Emission Maximum: ~596 nm
• Stokes Shift: ~15 nm
• High Quantum Yield: Provides bright and efficient fluorescence.
• Enhanced Solubility: Sulfonate groups improve water solubility, making it ideal for biological assays.

Applications of Sulfo-Cyanine3.5 Dye:

• Fluorescence Microscopy: Provides clear, high-resolution imaging of cells and tissues.
• Flow Cytometry: Offers distinct signals for precise cell sorting and analysis.
• Bioconjugation: Efficiently labels proteins, nucleic acids, and other biomolecules.
• Molecular Probes: Effective in bioanalytical assays to study molecular interactions.

Sulfo-Cy3.5’s bright fluorescence, excellent stability, and enhanced solubility make it a versatile tool for researchers needing reliable fluorescent labeling in various biological and biomedical applications.