Rhodamine Probe
Overview: Rhodamine probes are fluorescent dyes famous for their bright, vivid colors and versatility. They are used in various scientific and diagnostic applications, including imaging and labeling.
Key Features:
- Fluorescence Properties:
- Absorption Maximum: ~550-570 nm
- Emission Maximum: ~580-620 nm
- Quantum Yield: High, ensuring strong and clear fluorescence
- Chemical Properties:
- Polar: Rhodamine dyes are polar, which affects their interaction with biological molecules.
- Water-Soluble: Easily dissolves in water, making it suitable for use in aqueous solutions.
- Brightness and Stability:
- High Brightness: Offers intense fluorescence for accurate detection.
- Good Stability: Generally stable but can fade with prolonged exposure to light.
Applications:
- Fluorescence Microscopy: Great for visualizing cells, tissues, and other biological samples.
- Biochemical Assays: Used as a fluorescent tag in various assays, including enzyme and immunoassays.
- Cell Labeling: Ideal for tracking cells in flow cytometry and live cell imaging.
- Diagnostic Tests: Applied in clinical diagnostics to detect specific biomolecules.
Benefits:
- High Sensitivity: Provides bright and precise fluorescence for accurate results.
- Versatile: Suitable for many research and diagnostic applications.
- Water-Soluble: Works well in aqueous environments and biological systems.
Summary: Rhodamine probes are effective fluorescent dyes known for their bright and vivid colors. They are widely used in fluorescence microscopy, biochemical assays, and cell labeling, offering reliable performance and high sensitivity, though they may be sensitive to light exposure.
Ref:
Rhodamine-Based Fluorescent Probe for Highly Selective Determination of Hg2+ Nannan Zhu, Junhong Xu, Qiujuan Ma, Yang Geng, Linke Li, Shuzhen Liu, Shuangyu Liu, and Gege Wang ACS Omega 2022 7 (33), 29236-29245.
Englert, D., Burger, EM., Grün, F. et al. Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging. Nat Commun 14, 3879 (2023).
