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Both Q-TOF-LC-MS and TQ-LC-MS can be used for mass determination and quantification analysis of therapeutic oligonucleotides.

QTOF-LC-MS is a high-performance liquid chromatography (LC) tandem mass spectrometer (LC-MS/MS) combined with a quadrupole time-of-flight mass spectrometer (Q-TOF). High resolution TOF mass spectrometer can be used for quality characterization, quality control and quantification in the analysis of oligonucleotides.

Quantitative analysis of oligonucleotides can also be achieved by using a high-performance liquid chromatography-triple quadrupole mass spectrometer (TQ-LC-MS). TQ-LC-MS is an LC-MS/MS combining LC and a triple quadrupole mass spectrometer (TQ). It is possible to carry out selective and supersensitive analysis by multi-reaction monitoring (MRM), and it can be utilized for analysis of pharmacokinetics etc. in the analysis of therapeutic oligonucleotides.

When the difference between the target oligonucleotide and the impurities in the therapeutic agent is the structural difference only due to a slight modification, it is assumed to be undistinguishable by LC-UV detection because the chromatographic separation is difficult. Mass spectrometer based analysis, such as Q-TOF-LC-MS and TQ-LC-MS , on the other hand, are considered to be capable of highly selective detection due to their high mass resolution, mass accuracy, selectivity and sensitivity, even when chromatographic separation is not possible.

AxisPharm offers both TQ-LC-MS and QTOF-LC-MS for oligonucleotide analysis. We are fully engaged in providing oligonucleotide analysis services to support COVID-19 research for cure.