APDye Fluor 405 (AF405, Alexa Fluor 405 Equivalent) is a blue-fluorescent dye, equivalent to Alexa Fluor 405. It works with the 407 nm krypton and 408 nm violet lasers. The dye excites at 401 nm and emits at 421 nm. It is perfect for multicolor uses like flow cytometry and STORM microscopy. It stays stable from pH 4 to 10 and offers bright, reliable fluorescence.
APDye Fluor 405
APDye Fluor 405 (AF405, Alexa Fluor 405 Equivalent)
APDye Fluor 405 is a blue-fluorescent dye, optimized for use with the 407 nm krypton laser and 408 nm violet laser diode. It exhibits an excitation maximum at 401 nm and an emission maximum at 421 nm. This dye is pH-insensitive from pH 4 to pH 10, ensuring reliable performance across a range of conditions. It is ideal for multicolor applications, including flow cytometry and super-resolution microscopy (STORM).
Key Features:
- Alexa Fluor 405 Equivalent: Easy replacement for Alexa Fluor 405 in existing protocols.
- Laser Compatibility: Excites at 401 nm and emits at 421 nm, suited for krypton and violet lasers.
- pH Insensitive: Stable performance between pH 4 and pH 10.
- High Photostability: Resists fading, ensuring clear results during extended imaging.
- Bright Fluorescence: Strong signals with low background noise for precise detection.
Applications:
- Flow Cytometry: Ideal for multicolor assays and detecting multiple markers.
- Super-Resolution Microscopy (STORM): Suitable for nanoscale imaging.
- Fluorescence Microscopy: Provides clear visualization of proteins and cells.
- Immunofluorescence: Effective for labeling antibodies in immunoassays.
- Nucleic Acid Detection: Enhances sensitivity in DNA/RNA labeling.
- Protein Labeling: Great for tagging proteins in interaction and localization studies.
Storage and Handling:
Supplied in powder form. Store in a cool, dry place and protect from light.
Technical Specifications:
- Excitation Wavelength: 401 nm
- Emission Wavelength: 421 nm
- Laser Compatibility: Krypton (407 nm) and violet (408 nm)
- pH Stability: pH 4-10
- Form: Powder
Ordering Information:
Available in multiple sizes with custom conjugation options. Contact us for details and to place an order.