JOE is a green-fluorescent dye that is often used in molecular biology research for applications such as real-time PCR, genotyping, and sequencing.
JOE is a popular choice for many researchers due to its high quantum yield, which means that it produces a strong and efficient fluorescence signal. Additionally, JOE has a relatively narrow emission spectrum, which helps to minimize spectral overlap with other fluorescent probes, making it a useful tool for multiplexing experiments. JOE is also relatively insensitive to changes in pH, which makes it a stable and reliable fluorescent probe in various biological environments.