JOE
JOE (2′,7′-Dimethoxy-4′,5′-dichloro-6-carboxyfluorescein) is a green-fluorescent dye used in molecular biology for real-time PCR, genotyping, and sequencing. It delivers a strong fluorescence signal due to its high quantum yield, making it a go-to choice for researchers.
JOE’s narrow emission spectrum reduces overlap with other dyes, ideal for multiplexing. It remains stable across different pH levels, ensuring consistent fluorescence in various environments. This blend of brightness, stability, and precision makes JOE an excellent tool for sensitive detection in molecular biology.