SPDP-PEG links to sulfhydryl groups, forming stable but cleavable disulfide bonds. The PEG spacer increases solubility and reduces aggregation. Use it in bioconjugation, drug delivery, and diagnostics for efficient, reversible linking. Cleave the bond easily with DTT or other reducing agents when needed.
SPDP PEG
SPDP-PEG Overview
SPDP-PEG is a PEG-based crosslinker with a cleavable disulfide bond. The pyridyldithiol group reacts with reduced cysteines and other sulfhydryl groups, forming stable but easily cleavable disulfide linkages. The PEG spacer boosts solubility and stability, performing better than hydrocarbon spacers. You can break the disulfide bond using reducing agents like DTT.Key Features:
- Cleavable Disulfide Bond: Forms stable linkages that you can break with DTT or other reducing agents.
- PEG Spacer: Enhances solubility and reduces aggregation in biomolecules.
- Selective Conjugation: Reacts with sulfhydryl groups for precise linking.
Applications:
- Bioconjugation: Efficiently links proteins and peptides through disulfide bonds.
- Drug Delivery: Increases the solubility and stability of therapeutic agents.
- Diagnostics: Creates stable, functional conjugates for diagnostic testing.
Advantages:
- Better Solubility: The PEG spacer ensures higher solubility than traditional hydrocarbon spacers.
- Controlled Cleavage: Easily cleave disulfide bonds with DTT for flexible applications.
- Improved Stability: PEGylation enhances stability and reduces immune reactions.