APDye Fluor 555 (AF555, Alexa Fluor 555 equivalent) is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. APDye Fluor 555 has maximum excitation and emission wavelengths of 555/565 nm and can be excited efficiently using the 543 nm He-Ne laser line. APDye Fluor 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. APDye Fluor 555 molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
APDye Fluor 555 is superior to Cy3 alternatives, APDye Fluor 555 matches the spectrum of Cy3 conjugates and is therefore suitable for Cy3 filters. APDye Fluor 555 has higher total fluorescence and better photostability, providing researchers with longer image capture times. Learn more about Cy3b nhs ester, Cy3 azide, Cy3 nhs ester,etc.
APDye Fluor 555 antibody conjugates are suitable for a variety of applications, including flow cytometry, fluorescence microscopy, immunohistochemical techniques, primary detection, ELISA, immunocytochemical techniques, indirect FISH, and more.
