APDye Fluor 647 (Alexa Fluor 647 equivalent) is structurally similar to Alexa Fluor 647(AF647), and spectrally is almost identical to Cy5 Dye, Alexa Fluor 647, CF 647 Dye, or any other Cyanine5 based fluorescent dyes. APDye Fluor 647 is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. The dye is water soluble and pH-insensitive from pH 4 to pH 10. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. APDye Fluor 647 is used for protein and antibody labeling, or nucleic acid applications with high labeling density.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. APDye Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
We offer APDye Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.