Fluorescein Probe
Overview: Fluorescein probes are widely used fluorescent dyes known for their bright, strong fluorescence. They are valuable in research and diagnostics for various applications, including imaging and labeling.
Key Features:
- Fluorescence Properties:
- Absorption Maximum: ~495 nm
- Emission Maximum: ~515 nm
- Quantum Yield: High, providing vivid fluorescence
- Chemical Properties:
- Polar: Fluorescein is polar, which affects how it interacts with biological molecules.
- Water-Soluble: Easily dissolves in water, making it ideal for use in aqueous environments.
- Brightness and Stability:
- Bright Fluorescence: Offers strong, clear fluorescence for accurate detection.
- Moderate Stability: Can be prone to fading under intense light but is generally reliable for short-term use.
Applications:
- Fluorescence Microscopy: Used to visualize cells, tissues, and other biological samples.
- Biochemical Assays: Acts as a tag in enzyme activity and immunoassays.
- Cell Labeling: Useful for tracking cells in flow cytometry and live cell imaging.
- Diagnostic Tests: Applied in clinical diagnostics to detect specific biomolecules.
Benefits:
- High Sensitivity: Provides bright and accurate detection.
- Versatile: Suitable for a range of research and diagnostic applications.
- Water-Soluble: Works well in aqueous solutions and biological contexts.
Summary: Fluorescein probes are effective fluorescent dyes known for their bright, vivid fluorescence and water solubility. They are commonly used in microscopy, biochemical assays, and cell labeling, offering reliable performance though they may be sensitive to photobleaching.
Ref:
Fanyong Yan, Keqing Fan, Zhangjun Bai, Ruiqi Zhang, Fanlin Zu, Jinxia Xu, Xiang Li, Fluorescein applications as fluorescent probes for the detection of analytes, TrAC Trends in Analytical Chemistry, Volume 97,
2017, Pages 15-35.
Le Guern F, Mussard V, Gaucher A, Rottman M, Prim D. Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications. Int J Mol Sci. 2020 Dec 3;21(23):9217. doi: 10.3390/ijms21239217. PMID: 33287208; PMCID: PMC7729466.
