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APDye Fluor 488 (AF488 or Alexa Fluor 488 equivalent) is a bright green, water-soluble fluorescent dye optimized for 488 nm lasers. It provides stable fluorescence across a wide pH range, making it perfect for imaging and flow cytometry. AF488 offers high labeling efficiency with minimal quenching, suitable for conjugating to antibodies, proteins, peptides, and other biomolecules. Available in various conjugates, AF488 is ideal for diverse bioconjugation applications, including click chemistry and thiol-reactive conjugations.

APDye Fluor 488

Cat# Name Structure M.W. Purity Pricing
AP12415APDye 488 Acid534.47 (protonated)≥95% Pricing
AP13835APDye 488 Hydrazide548.50 (protonated) ≥95% Pricing
AP12414APDye 488 Hydroxylamine606.58 (protonated)≥95% Pricing
AP13944APDye 488 Tyramide653.63 (protonated)≥95% Pricing
AP12416APDye 488 TFP ester682.53 (protonated)≥95% Pricing
AP12632APDye 488 Tetrazine703.66≥95% Pricing
AP12417APDye 488 Maleimide720.66≥95% Pricing
AP15231APDye 488 amine733.87≥95% Pricing
AP12663APDye 488 Azide Plus745.75 (protonated)≥95% Pricing
AP12630APDye 488 TCO770.82≥95% Pricing
AP10632APDye 488 Alkyne773.92≥95% Pricing
AP12818APDye 488 C3 azide818.96≥ (95%) Pricing
AP10629APDye 488 Cadaverine821.02≥95% Pricing
AP10627APDye 488 NHS ester (Alexa Fluor® 488 NHS equivalent)833.93≥95% Pricing
AP12819APDye 488 NHS ester833.93≥ (80+%, balance mostly carboxylic acid) Pricing
AP10630APDye 488 Azide C5847.02≥95% Pricing
AP13834APDye 488 Azide861.04 ≥95% Pricing
AP10626AFDye 488 TFP ester884.91≥95% Pricing
AP10628AFDye 488 Maleimide C6734.683≥95% Pricing
AP13861APDye 488 Gly-Gly-Gly907.92≥95% Pricing
AP10631APDye 488 Picolyl Azide924.06≥95% Pricing
AP12642APDye 488 Biotin935.00 (protonated)≥95% Pricing
AP12418APDye 488 DBCO995.18≥95% Pricing

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    APDye Fluor 488 (AF488 or Alexa Fluor 488 equivalent) – Bright Green Fluorescent Dye

    Overview: APDye Fluor 488 (AF488) is a water-soluble, green-fluorescent dye that’s perfect for use with a 488 nm laser. It delivers bright, stable fluorescence that is not affected by pH changes, making it ideal for imaging and flow cytometry applications.

    Key Features:

    • Fluorescence Properties:
      • Color: Bright green
      • Laser Line: Optimized for 488 nm
      • pH Stability: Consistent fluorescence from pH 4 to pH 10
      • High Labeling Efficiency: Achieve high levels of labeling with minimal quenching
    • Versatility:
      • Applications: Can be conjugated to antibodies, peptides, proteins, tracers, and amplification substrates
      • Uses: Perfect for both imaging and flow cytometry

    Available Conjugates:

    AxisPharm provides a variety of APDye Fluor 488 conjugates, including:

    • APDye-488-NHS: For amine coupling
    • APDye-488-amine: Direct amine conjugation
    • APDye-488-DBCO: For click chemistry applications
    • APDye-488-BCN: Another option for click chemistry
    • APDye-488-azide: For click chemistry and labeling
    • APDye-488-maleimide: For thiol-reactive conjugations

    Bioconjugation Kits:

    We also offer APDye-488 bioconjugation kits to simplify the process of conjugating the dye with your target molecules.

    Benefits:

    • Bright and Stable: Provides strong, clear fluorescence for accurate results.
    • pH Stable: Maintains performance across a wide pH range.
    • Efficient Labeling: Allows for high levels of labeling with minimal signal loss.

    Summary: APDye Fluor 488 (AF488) is an excellent choice for green fluorescence applications. It offers bright, stable fluorescence and is compatible with a range of conjugates and bioconjugation kits, making it ideal for imaging and flow cytometry.

    Ref:

    Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB. Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions. Anal Biochem. 2002 Sep 1;308(1):18-25.