Label-Free Protein Quantification and Identification Services

At AxisPharm, we specialize in label-free protein quantification and identification using advanced mass spectrometry. This technique offers a fast and efficient way to measure protein levels and identify proteins in your samples—without the need for labels or tags. It’s a reliable method for analyzing complex biological systems.

What is Label-Free Protein Quantification?

Label-free protein quantification measures protein abundance without using any chemical labels. Instead, mass spectrometry tracks protein levels based on peptide signals or spectral counts. This method is perfect for:

  • Comparing protein levels between different conditions
  • Tracking changes in protein expression
  • Identifying proteins in complex mixtures

Why Use Label-Free Protein Quantification?

Differences-between-label-free-quantification-and-Labeled-quantification

Differences-between-label-free-quantification-and-Labeled-quantification

  1. No Labeling Needed:
    This method skips the labeling step, making it faster and simpler.

  2. Cost-Effective:
    You avoid the added expense of labeling reagents while still getting high-quality results.

  3. Ideal for Complex Samples:
    It works well with complex samples like tissues, cell lysates, or biofluids.

  4. Accurate and Sensitive:
    By using peptide intensity or spectral counts, this technique gives you precise and sensitive measurements of protein abundance.

How Does Label-Free Protein Quantification Work?

  1. Sample Preparation:
    We extract proteins from your samples and digest them into peptides, ready for mass spectrometry.

  2. LC-MS/MS Analysis:
    Using advanced LC-MS/MS, we analyze peptides to identify and quantify proteins. We rely on peptide ion intensities or spectral counts to measure protein levels.

  3. Data Analysis:
    We compare protein intensities across different samples to show changes in protein levels. Our software identifies the proteins based on the peptide data.

  4. Report Generation:
    We provide a clear report detailing the identified proteins, their abundance, and statistical comparisons across samples.

Key Features of Our Label-Free Protein Quantification Service

  • Comprehensive Protein Identification:
    We identify and quantify proteins from a variety of sample types, such as cell lysates, tissues, and biofluids.

  • Accurate Quantification:
    Our label-free method ensures reliable and accurate measurements of protein levels.

  • High Throughput:
    We handle large-scale projects and can analyze hundreds or thousands of proteins in a single run.

Applications of Label-Free Protein Quantification

  1. Biomarker Discovery:
    Identify proteins that are differentially expressed between healthy and diseased samples.

  2. Drug Development:
    Track how protein levels change in response to drug treatments.

  3. Proteomics Research:
    Study protein expression profiles under various conditions or time points.

  4. Pathway Analysis:
    Discover key proteins involved in specific pathways or biological processes.

Sample Requirements

  • Protein Purity: At least 85%
  • Sample Quantity: 20-50 µg per sample
  • Buffer: Avoid high salt concentrations and detergents
  • Protein Extraction: Clean extraction methods will ensure better results.

Technologies We Use

At AxisPharm, we use cutting-edge LC-MS/MS systems, including the ThermoFisher Fusion Lumos and Q-Exactive Plus, to deliver high-resolution, high-sensitivity protein quantification and identification.

Deliverables

  • A detailed report that includes:

    • Identified proteins and associated peptides
    • Relative quantification data comparing protein levels across samples
    • Statistical analysis of protein changes between conditions

  • An Excel file or HTML report with:

    • Complete protein identification and quantification data
    • Clear comparisons between different sample groups

Turnaround Time: 2-4 weeks

Contact Us

Ready to start your label-free protein quantification project? Contact us today to see how AxisPharm’s mass spectrometry services can support your research needs!

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    Ref:

    Deracinois, Barbara & Flahaut, Christophe & Duban-Deweer, Sophie & Karamanos, Yannis. (2013). Comparative and Quantitative Global Proteomics Approaches: An Overview. Proteomes. 1. 180-218. 10.3390/proteomes1030180.