DTSSP crosslinker is a type of small molecule linker that is widely used in biochemistry and molecular biology research. It belongs to the category of homo-bifunctional crosslinkers, which means that it contains two reactive functional groups of the same type. DTSSP crosslinker is composed of several functional groups, including a 3,3′-dithiobis(sulfosuccinimidyl propionate) (DTSSP) group, which is the reactive functional group, and a spacer arm that connects the two DTSSP groups.
DTSSP crosslinker is widely used to study protein-protein interactions and to map protein structures. It works by covalently linking two or more proteins or other biomolecules together, allowing researchers to study their interactions and functions. The DTSSP group reacts with primary amines on the surface of proteins to form a stable crosslink, which can then be analyzed using a variety of biochemical and biophysical techniques.