PEG NHS ester linkers have been widely usded for crosslinking or labeling peptides and proteins. They readily react with primary amine groups at the N-terminus of polypeptide chains and lysine amino groups. These primary amines exist predominantly on the outside surfaces of protein and are readily accessible to NHS ester for bioconjugations. In addition, these primary amines are nucleophilic enough to react with PEG NHS esters for protein crosslinking and labeling.
The functional groups on the other end of the linkers provide protein scientist opportunities for further conjugation with other biomolecules or drug targets.
Incorporating a PEG spacer can dramatically improve the solubility of the linker and make them ideal for ADC conjugations as most of the process take place in aqueous media.
AxisPharm maintain a large collection of high quality PEG NHS ester linkers for our clients to select. For custom synthesis of ADC linkers, please contact us.