TCO-PEG Overview
TCO-PEG Linkers are essential bioorthogonal agents for
tetrazine reactions. The
TCO-tetrazine cycloaddition happens at an ultrafast speed, making it highly effective for
bioconjugation,
molecular imaging, and
cell-based diagnostics. This reaction stands out with unmatched kinetics in bioorthogonal chemistry.
Hydrophobic
TCOs often lose activity due to interactions with monoclonal antibodies (mAbs). However, adding
PEG spacers minimizes these interactions. The
PEGylation improves solubility, reduces aggregation, and enhances pharmacokinetic and pharmacodynamic properties.
Key Features:
- Ultrafast Ligation: TCO-tetrazine reactions happen quickly and efficiently.
- PEG Spacers: Increase solubility and reduce unwanted interactions with mAbs.
- Enhanced Stability: PEGylation boosts the pharmacokinetics and reduces immunogenicity.
Applications:
- Bioconjugation: Perfect for quick and selective labeling of proteins and peptides.
- Molecular Imaging: Ideal for precise, fast molecular tracking in imaging.
- Cell Diagnostics: Efficient for use in cell-based diagnostic applications.
Advantages:
- Rapid Reactions: TCO-tetrazine ligation offers the fastest bioorthogonal chemistry available.
- Improved Solubility: PEGylation increases water solubility and reduces mAb aggregation.
- Better Drug Delivery: PEG boosts the pharmacokinetics and reduces immune response for enhanced therapeutic effects.
TCO-PEG provides a powerful, fast solution for researchers in
bioconjugation,
imaging, and
drug development. Its combination of rapid reaction and PEG benefits ensures effective and reliable results.
Ref:
García-Maceira T, García-Maceira FI, González-Reyes JA, Torres-Sánchez LA, Aragón-Gómez AB, García-Rubiño ME, Paz-Rojas E.
Covalent Immobilization of Antibodies through Tetrazine-TCO Reaction to Improve Sensitivity of ELISA Technique. Biosensors (Basel). 2021 Dec 20;11(12):524. 
