TCO-NHS Acetate is a commonly used reagent in bioconjugation reactions for the covalent attachment of TCO (trans-cyclooctene) to biomolecules such as proteins, peptides, and antibodies. The reagent contains an NHS (N-hydroxysuccinimide) ester functional group, which is a commonly used reactive group in bioconjugation.
TCO is an alkene with a strained cyclooctene ring structure that undergoes a rapid and selective reaction with a complementary functional group, tetrazine (Tz), in a bioorthogonal “click” reaction. This reaction proceeds quickly and with high specificity, even in complex biological systems, making it an attractive option for in vivo imaging and therapeutic applications.
TCO-NHS Acetate can be easily used to introduce TCO groups onto biomolecules via an amide bond formation reaction with primary amines. The resulting TCO-modified biomolecules can then be conjugated with Tz-functionalized imaging probes or therapeutic agents, enabling efficient and specific targeting of cells or tissues of interest.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
