Phosphoramidites are essential building blocks in oligonucleotide synthesis, driving the creation of custom DNA or RNA sequences. They feature a reactive phosphate group, enabling precise stepwise addition of nucleotides. This method ensures efficient and controlled synthesis in various research and therapeutic applications.
Key Features:
Reactive Phosphate Group: Enables efficient bonding in nucleic acid synthesis.
High Coupling Efficiency: Ensures rapid and precise nucleotide addition.
Selective Protection: Uses 5′-O-DMTr to control reactions, preventing unwanted outcomes.
Applications:
Oligonucleotide Synthesis: Ideal for producing custom DNA or RNA strands.
Therapeutic Development: Crucial for designing antisense oligonucleotides and siRNA.
Gene Synthesis: Helps construct synthetic genes for research and therapies.
Advantages:
Precision: Facilitates highly controlled synthesis of nucleotides.
Versatility: Suitable for both DNA and RNA, with room for modifications.
Phosphoramidites offer researchers in nucleic acid research and therapeutic development a reliable, high-precision method for synthesizing custom oligonucleotides.