DMT-5Me-dC(Bz)-CE Phosphoramidite

5′-O-DMT-N4-Benzoyl-2′-Deoxy-5-Methylcytidine-CE Phosphoramidite (CAS: 105931-57-5)

5′-O-DMT-N4-Benzoyl-2′-Deoxy-5-Methylcytidine-CE Phosphoramidite (CAS: 105931-57-5) is a nucleotide phosphoramidite used for oligonucleotide synthesis. It has a protective 5′-O-DMT group, an N4-benzoyl modification, and a 5-methylcytidine base.

Key Features:

• 5′-O-DMT Group: Protects the 5′ hydroxyl for selective coupling.
• N4-Benzoyl Group: Stabilizes the amine at the N4 position.
• 5-Methylcytidine Base: Enhances stability and binding affinity.

Applications:

• Oligonucleotide Synthesis: Produces modified DNA/RNA sequences.
• Gene Expression Studies: Used in probes and primers for gene analysis.
• Antisense Oligonucleotides: Increases stability for therapeutic use.
• Diagnostics: Supports PCR and hybridization assays.

This phosphoramidite is vital for synthesizing modified oligonucleotides in various molecular biology applications.