DMT-dC(ac) Phosphoramidite

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5′-O-DMT-N4-Acetyl-2′-Deoxycytidine-CE Phosphoramidite | CAS: 154110-40-4

Overview:

5′-O-DMT-N4-Acetyl-2′-Deoxycytidine-CE Phosphoramidite (CAS: 154110-40-4) is a high-purity reagent essential for the solid-phase synthesis of DNA oligonucleotides. This specialized compound incorporates deoxycytidine bases into DNA sequences, ensuring precise and efficient nucleotide addition. The protective groups, such as DMT and N4-acetyl, safeguard reactive sites, preventing unwanted side reactions during the synthesis process.

Applications:

1. DNA Oligonucleotide Synthesis:
   Ideal for use in automated DNA synthesizers, this phosphoramidite plays a crucial role in creating custom oligonucleotides for applications such as PCR, gene editing, and molecular diagnostics.
2. Research and Diagnostics:
   Commonly used in the synthesis of primers, probes, and antisense oligonucleotides, it is a key component in molecular biology research and diagnostic assays.

Key Features:

• Protective Groups:
  The DMT (dimethoxytrityl) group protects the 5’-hydroxyl position, while the N4-acetyl group safeguards the amino group on cytidine, ensuring accurate synthesis and preventing side reactions.
• High Purity:
  Offers high-purity synthesis, guaranteeing the precision and efficiency needed for complex oligonucleotide projects.

Storage and Handling:

• Storage:
  To maintain stability, store this compound under dry, inert conditions such as in a nitrogen or argon environment.
• Handling:
  Handle using standard laboratory protocols for moisture-sensitive reagents, ensuring the compound remains intact for optimal performance.

Why Choose 5′-O-DMT-N4-Acetyl-2′-Deoxycytidine-CE Phosphoramidite?

This phosphoramidite is a critical reagent in synthetic biology, genetic research, and the development of DNA-based therapeutic agents. Its reliability in custom DNA synthesis makes it the go-to choice for high-precision applications.

FAQs:

What is 5′-O-DMT-N4-Acetyl-2′-Deoxycytidine-CE Phosphoramidite used for?
This phosphoramidite is primarily used in the solid-phase synthesis of custom DNA sequences, particularly in gene editing and PCR technologies.

How should I store this product?
Store it in a dry, inert atmosphere to prevent moisture from affecting its stability and reactivity.

Is this phosphoramidite suitable for large-scale DNA synthesis projects?
Yes, it is optimized for use in both small and large-scale oligonucleotide synthesis, making it suitable for high-throughput labs.