Amino-Modifier C6 dT provides an amino-functionalized thymidine building block for DNA oligonucleotide synthesis and post-synthetic bioconjugation and specifically combines a deoxythymidine nucleoside with a six-carbon aminoalkyl linker for introducing primary amine functionality at selected positions within synthetic DNA. This modification specifically supports fluorescent labeling, biotin conjugation, probe development, and functionalized nucleic acid research.
Key Features and Benefits
- Amino-Modified Thymidine: Features a deoxythymidine-based structure with an aminoalkyl substituent for introducing functional groups into synthetic DNA sequences.
- Six-Carbon Linker: Provides a flexible C6 spacer that separates the reactive amino group from the oligonucleotide backbone.
- Primary Amine Functionality: Generates a reactive amino group after appropriate deprotection for conjugation with compatible labeling reagents.
- Site-Specific DNA Modification: Enables controlled incorporation of amino-functionalized thymidine at selected positions during oligonucleotide synthesis.
- Phosphoramidite Synthesis Compatibility: Supports incorporation into growing DNA chains when supplied in the appropriate phosphoramidite form.
- Post-Synthetic Labeling: Enables attachment of fluorescent dyes, biotin derivatives, and other amine-reactive molecules after oligonucleotide synthesis.
- Flexible Spacer Design: The C6 linker provides separation between DNA and attached functional groups, supporting conjugation and molecular recognition studies.
- NHS Ester Coupling Compatibility: Supports formation of stable amide bonds with NHS ester-functionalized dyes, affinity tags, and other reagents.
- Versatile DNA Functionalization: Enables preparation of modified oligonucleotides for molecular detection, affinity capture, and biomolecular interaction studies.
Applications
- Modified DNA Oligonucleotide Synthesis: Supports incorporation of amino-functionalized thymidine residues into synthetic DNA sequences.
- Fluorescent DNA Labeling: Enables attachment of fluorescent dyes through reactions between deprotected amino groups and suitable labeling reagents.
- DNA Probe Development: Supports preparation of functionalized oligonucleotide probes for hybridization and molecular detection applications.
- Biotinylated DNA Preparation: Enables conjugation of biotin derivatives for streptavidin-based capture and affinity studies.
- Post-Synthetic Bioconjugation: Provides primary amine functionality for coupling DNA oligonucleotides with compatible biomolecules and chemical modifiers.
- Nucleic Acid Biosensors: Supports development of chemically modified DNA probes for fluorescence-based and affinity-based detection systems.
- DNA Immobilization: Enables covalent attachment of modified oligonucleotides to NHS ester-activated surfaces and compatible solid supports.
- DNA Nanotechnology: Supports incorporation of functional attachment sites into DNA nanostructures and molecular assemblies.
- Molecular Interaction Studies: Useful for preparing labeled DNA constructs for investigating hybridization, protein binding, and molecular recognition.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: For phosphoramidite derivatives, handle under anhydrous conditions and prepare solutions using dry, compatible solvents, such as anhydrous acetonitrile. Minimize atmospheric moisture exposure during oligonucleotide synthesis. Following synthesis, remove amino protecting groups using validated deprotection conditions. React the liberated primary amine with compatible NHS esters or other amine-reactive reagents under suitable coupling conditions.
Amino-Modifier C6 dT provides a flexible aminoalkyl linker for introducing reactive functionality into synthetic DNA oligonucleotides and specifically supports fluorescent labeling, biotin conjugation, DNA immobilization, molecular probe development, and advanced nucleic acid research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
