2-O-DMT-Sulfonyldiethanol phosphoramidite provides a protected sulfonyl-containing phosphoramidite building block for oligonucleotide synthesis and nucleic acid modification research and specifically combines a sulfonyldiethanol framework with a dimethoxytrityl (DMT)-protected hydroxyl group and a reactive phosphoramidite functionality. This non-nucleosidic reagent specifically supports incorporation of sulfone-containing linkers into synthetic oligonucleotides and functionalized molecular constructs.
Key Features and Benefits
- Sulfonyldiethanol Framework: Features a sulfone-containing organic linker with oxygen-containing functional groups for incorporation into modified molecular structures.
- DMT-Protected Hydroxyl Group: Provides a dimethoxytrityl-protected hydroxyl functionality specifically for controlled extension during solid-phase oligonucleotide synthesis.
- Reactive Phosphoramidite Group: Supports coupling with hydroxyl-containing substrates using compatible phosphoramidite activation chemistry.
- Non-Nucleosidic Building Block: Enables introduction of a functional linker without adding a conventional nucleobase to the oligonucleotide sequence.
- Sulfone-Containing Linker: Incorporates a polar sulfonyl group specifically for investigating linker composition, molecular interactions, and physicochemical properties.
- Controlled Solid-Phase Synthesis: Supports sequential coupling, oxidation, and detritylation steps using appropriately optimized synthesis conditions.
- Functional Spacer Design: Provides a flexible organic spacer specifically for connecting oligonucleotide segments or incorporating non-nucleotide structural elements.
- Chemical Modification Compatibility: Supports preparation of modified oligonucleotides and functionalized constructs through established phosphoramidite chemistry.
- Versatile Research Applications: Enables investigation of linker chemistry, oligonucleotide architecture, and structure-property relationships.
Applications
- Modified Oligonucleotide Synthesis: Supports incorporation of sulfone-containing non-nucleosidic linkers into synthetic DNA and other compatible oligonucleotide constructs.
- Oligonucleotide Spacer Incorporation: Enables introduction of a functional spacer between nucleotide segments or other molecular components.
- Solid-Phase Phosphoramidite Chemistry: Useful for developing and optimizing coupling protocols involving non-nucleosidic phosphoramidite reagents.
- Nucleic Acid Probe Development: Supports preparation of modified probes containing sulfone-based linker structures.
- DNA Linker Research: Enables investigation of non-nucleotide spacers and their effects on oligonucleotide architecture and molecular properties.
- Functional Oligonucleotide Design: Supports development of synthetic oligonucleotides containing additional structural elements beyond conventional nucleotides.
- Bioconjugation Precursor Synthesis: Useful for preparing linker-containing intermediates for subsequent chemical modification and conjugation.
- Nucleic Acid Materials Research: Supports investigation of functionalized oligonucleotides for molecular assemblies and biomaterial applications.
- Linker Structure Studies: Enables evaluation of sulfone-containing spacers, molecular flexibility, and chemical stability in synthetic constructs.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle under anhydrous conditions to preserve phosphoramidite reactivity. Prepare solutions using dry, compatible solvents, such as anhydrous acetonitrile, when appropriate. Minimize atmospheric moisture exposure and use freshly prepared solutions. Apply validated coupling, oxidation, and detritylation conditions compatible with the sulfonyldiethanol framework.
2-O-DMT-Sulfonyldiethanol phosphoramidite combines a protected hydroxyl group with reactive phosphoramidite functionality for controlled incorporation of sulfone-containing linkers and specifically supports modified oligonucleotide synthesis, functional spacer development, nucleic acid probe preparation, and advanced phosphoramidite chemistry research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
