DMT-dG(iBu) Phosphoramidite (Isobutyryl-Protected Deoxyguanosine) provides a protected deoxyguanosine building block for solid-phase DNA oligonucleotide synthesis. It features a 5′-dimethoxytrityl (DMT) protecting group, an N²-isobutyryl-protected guanine base, and a reactive 3′-phosphoramidite group. This reagent specifically supports controlled nucleotide coupling, sequence-specific DNA synthesis, and the development of synthetic oligonucleotides.
Key Features and Benefits
- Protected Deoxyguanosine Building Block: Provides a guanine nucleotide component for incorporating deoxyguanosine residues into synthetic DNA sequences.
- 5′-DMT Protecting Group: Protects the 5′-hydroxyl group during synthesis and enables controlled deprotection for sequential nucleotide addition.
- N²-Isobutyryl Protection: Protects guanine’s exocyclic amino group during oligonucleotide assembly, helping minimize unwanted side reactions.
- Reactive 3′-Phosphoramidite: Enables efficient nucleotide coupling through established phosphoramidite chemistry under suitable activation conditions.
- DNA Synthesis Compatibility: Supports automated and manual solid-phase DNA synthesis using compatible coupling, capping, oxidation, and deprotection protocols.
- Controlled Nucleotide Incorporation: Enables stepwise addition of deoxyguanosine residues during sequence-directed DNA oligonucleotide assembly.
- Cyanoethyl Phosphate Protection: Features a conventional 2-cyanoethyl protecting group that protects the phosphate functionality during synthesis.
- High Synthetic Versatility: Supports preparation of DNA oligonucleotides with defined guanine positions for molecular biology and biochemical research.
- Established Protecting Group Strategy: Combines DMT and isobutyryl protection to support controlled synthesis and subsequent oligonucleotide deprotection.
Applications
- DNA Oligonucleotide Synthesis: Supports incorporation of protected deoxyguanosine residues into DNA sequences through solid-phase phosphoramidite chemistry.
- Automated DNA Synthesis: Enables use in compatible automated oligonucleotide synthesizers for controlled, sequence-specific DNA chain assembly.
- Custom DNA Sequence Development: Supports preparation of research-grade DNA oligonucleotides with defined nucleotide sequences and compositions.
- PCR Primer Synthesis: Provides a deoxyguanosine building block for synthesizing DNA primers used in polymerase chain reaction research.
- DNA Probe Development: Supports preparation of sequence-specific DNA probes for hybridization, molecular detection, and analytical applications.
- Antisense Oligonucleotide Research: Enables synthesis of DNA-containing oligonucleotides for investigating complementary nucleic acid interactions and gene regulation.
- DNA Structure Studies: Supports preparation of defined DNA sequences for investigating secondary structures, duplex stability, and molecular interactions.
- Modified Oligonucleotide Development: Enables incorporation of deoxyguanosine residues into experimental DNA sequences containing compatible nucleotide modifications.
- Nucleic Acid Chemistry Research: Supports investigations of phosphoramidite coupling, protecting group strategies, and oligonucleotide synthesis optimization.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container, protected from moisture and light. Follow product-specific storage instructions when available. Minimize exposure to atmospheric humidity to preserve phosphoramidite reactivity.
- Handling: Handle using clean, dry equipment under moisture-controlled conditions. Prepare solutions in anhydrous acetonitrile or another validated synthesis solvent. Confirm solubility and compatibility with the selected oligonucleotide synthesis system.
- Use the phosphoramidite in solid-phase DNA synthesis with a compatible activator and coupling protocol. Optimize reagent concentration, coupling time, and synthesis conditions for the desired DNA sequence. Protect the reagent from moisture because phosphoramidite groups undergo hydrolysis.
- Maintain appropriate protecting group strategies throughout DNA chain assembly. Remove the 5′-DMT group during the detritylation step to expose the hydroxyl group for subsequent coupling. The N²-isobutyryl group protects guanine during synthesis and requires compatible final deprotection conditions.
- Evaluate coupling efficiency, oligonucleotide integrity, and final DNA purity using appropriate analytical techniques. Apply validated cleavage and deprotection procedures after synthesis. Remove residual reagents and protecting group byproducts before downstream applications. Avoid prolonged exposure to moisture, strong acids, and excessive heat. Confirm the product’s chemical identity, purity, and phosphoramidite specifications before use.
DMT-dG(iBu) Phosphoramidite (Isobutyryl-Protected Deoxyguanosine) provides a protected guanine building block for controlled DNA oligonucleotide synthesis and specifically supports automated DNA synthesis, custom primer development, nucleic acid probe preparation, and advanced oligonucleotide research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
