TCO Linkers are bioorthogonal agents for tetrazine. The cycloaddition reaction between tetrazine and trans-cyclooctene (TCO) is rapidly growing in use for bioconjugation, molecular imaging and cell-based diagnostics.
The chemoselective TCO–Tz ligation pair possess ultrafast kinetics unmatched by any other bioorthogonal ligation pair.
Hydrophobic TCOs can be masked to lose activity by hydrophobic interactions with mAb. However, pegylating TCOs with polyethylene glycol (PEG) spacers help minimize the interaction and improve the activity. In addition, PEG spacers help reduce aggregation of mAb conjugates and provide increased water solubility, reduced immunogenicity, improved pharmacokinetics and pharmacodynamics properties.
AxisPharm has large production capacity for high quality TCO PEG linkers with amine, aminooxy, NHS ester and acid functionality. We also provide custom synthesis of TCO PEG modified folate, biotin and fluorescent dye for biological applications.