Cholesterol-PEG-Folate (CLS-PEG-FA,Cholesterol-PEG-FA,Cholesterol-PEG-Folic acid) is a luteal, folate-functionalized polyethylene glycol cholesterol that is lipophilic and soluble in organic solvents and aqueous solutions. PEG cholesterol derivatives can be used to prepare targeted liposomes to improve the circulation time of encapsulated drugs. They can also be used in non-viral transfection reagents.