Cy5 Labeling uses the Cy5 fluorescent dye, which emits bright far-red light. It has an absorption maximum at 649 nm and an emission maximum at 670 nm. Cy5 is ideal for tagging proteins, nucleic acids, and other biomolecules. It enhances sensitivity and reduces background interference in imaging and detection.
Cyanine5 Dye
Cyanine 5 Dye (Cy5)
Overview: Cyanine 5 (Cy5) is a fluorescent dye renowned for its bright and stable fluorescence. It is widely used in various scientific applications, including imaging and molecular biology.
Key Features:
Fluorescence Properties:
Absorption Maximum: ~649 nm
Emission Maximum: ~670 nm
Quantum Yield: High, around 0.28
Brightness: Cy5 is highly fluorescent, making it easy to detect even at low concentrations.
Stability: It is very stable and resists fading (photobleaching) during extended use.
Applications:
Molecular Imaging: Ideal for fluorescence microscopy to visualize biological samples.
Flow Cytometry: Used for analyzing cells and particles in flow cytometers.
Western Blotting: Helps in detecting and quantifying proteins on blots.
Fluorescent Probes: Serves as a tag in various assays, including ELISA and FRET.
Benefits:
High Sensitivity: Produces strong signals for precise detection and quantification.
Versatile Use: Suitable for a range of research and clinical applications.
Durable: Maintains performance over time, reducing the need for frequent re-testing.
Summary: Cyanine 5 Dye (Cy5) is a versatile and reliable fluorescent dye, offering strong and stable fluorescence for enhanced imaging and detection in scientific research.
Ref:
Ma, X., Shi, L., Zhang, B. et al. Recent advances in bioprobes and biolabels based on cyanine dyes. Anal Bioanal Chem 414, 4551–4573 (2022).