RAPID DiO (FAST DiO™ or Dilinoleyl DiO) is a green-fluorescent, lipophilic tracer designed for fast and effective cell membrane labeling. Its unsaturated hydrocarbon chains enable faster diffusion within lipid bilayers compared to traditional DiO, making it ideal for neuronal tracing and membrane studies in both live and fixed cells.
Key Features:
- Fast Diffusion: Moves about 50% faster in membranes than conventional DiO, enabling quicker labeling.
- Stable Membrane Integration: Inserts deeply into lipid bilayers, ensuring long-lasting and even staining.
- Minimal Transfer: Stays localized in labeled cells with little transfer to neighboring cells.
RAPID DiO Applications:
- Neuronal Tracing: Works as both an anterograde and retrograde tracer for mapping neuronal networks.
- Cell Membrane Labeling: Stains membranes in various cell types for morphology and dynamic studies.
- Dual-Color Imaging: Combines well with other dyes like RAPID DiI for multi-color fluorescence imaging.
Handling and Storage:
- Solubility: Dissolves in ethanol, methanol, and DMSO for easy solution preparation.
- Storage: Keep at -20°C, away from light, to maintain stability.
RAPID DiO’s fast labeling, strong membrane affinity, and compatibility with other dyes make it a top choice for researchers studying cell membranes, neuronal pathways, and tissue structures.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
