Fmoc-D-Lys(Biotin-PEG12)-OH is a biotinylated D-lysine derivative designed for solid-phase peptide synthesis (SPPS), site-specific peptide biotinylation, and affinity-based research. It features an Fmoc-protected α-amino group and a biotin moiety attached to the lysine side chain through a hydrophilic PEG12 spacer. Additionally, its D-configuration supports incorporation of D-amino acids into functionalized peptides for biochemical and molecular recognition studies.
Key Features and Benefits
- Fmoc-Protected D-Lysine: Features an Fmoc-protected α-amino group compatible with standard Fmoc-based peptide synthesis.
- Biotin-PEG12 Functionalization: Contains a biotin moiety connected through a 12-unit polyethylene glycol spacer for peptide biotinylation.
- D-Amino Acid Configuration: Provides D-lysine stereochemistry for preparing peptides containing noncanonical stereochemical configurations.
- Hydrophilic PEG12 Spacer: Improves linker flexibility and hydrophilicity while separating the biotin group from the peptide backbone.
- Streptavidin Binding Capability: Enables strong noncovalent interactions with streptavidin and avidin for affinity-based applications.
- Solid-Phase Peptide Synthesis Compatibility: Supports incorporation into peptide sequences using conventional Fmoc-SPPS coupling and deprotection protocols.
- Site-Specific Biotin Incorporation: Specifically enables biotin placement at selected lysine positions during peptide assembly.
- Free Carboxylic Acid Group: Provides a reactive carboxyl group for peptide bond formation with suitable amino-functionalized substrates.
- Research Versatility: Additionally, supports preparation of biotinylated peptides for affinity purification, immobilization, and molecular interaction assays.
Applications
- Solid-Phase Peptide Synthesis: Enables incorporation of biotin-PEG12-functionalized D-lysine into peptide sequences using Fmoc-SPPS.
- Site-Specific Peptide Biotinylation: Supports controlled introduction of biotin groups at defined positions within synthetic peptides.
- Streptavidin-Based Affinity Assays: Facilitates peptide capture and detection using streptavidin- or avidin-containing assay systems.
- Peptide Immobilization: Enables attachment of biotinylated peptides to streptavidin-coated surfaces, beads, and analytical platforms.
- Affinity Purification: Specifically supports isolation of biotinylated peptide conjugates through streptavidin-based affinity methods.
- Protein–Peptide Interaction Studies: Facilitates investigation of molecular binding interactions using immobilized or labeled biotinylated peptides.
- D-Peptide Research: Supports preparation of D-amino-acid-containing peptides for studies of stereochemistry and peptide recognition.
- Biosensor Development: Enables preparation of biotinylated peptide probes for streptavidin-based biosensing platforms.
- Biochemical Assay Development: Additionally, supports development of peptide-based binding assays, molecular recognition studies, and affinity detection methods.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container, protected from moisture and light, unless product-specific documentation indicates otherwise. Additionally, minimize repeated temperature fluctuations to maintain reagent stability.
- Handling: Allow the sealed container to reach room temperature before opening. Use clean, dry equipment and prepare solutions in a compatible solvent, such as DMF or DMSO, after confirming solubility.
- Peptide Synthesis: Incorporate the derivative using standard Fmoc-SPPS coupling conditions. Subsequently, remove the Fmoc protecting group under suitable basic conditions and continue peptide chain assembly.
- Bioconjugation and Analysis: Purify synthesized peptides using appropriate chromatography methods. Confirm peptide identity and purity by HPLC and mass spectrometry, and evaluate biotin accessibility using suitable streptavidin-binding assays.
Fmoc-D-Lys(Biotin-PEG12)-OH combines Fmoc-protected D-lysine with a flexible biotin-PEG12 linker for controlled peptide functionalization. Its structure supports site-specific biotin incorporation, while its PEG spacer improves accessibility for streptavidin-based interactions. Therefore, it provides a versatile building block for peptide synthesis, affinity assays, peptide immobilization, and biochemical research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
