(E,E)-3,5-Bis(phenylethenyl)-BDP Lipid Stain is a red fluorescent BODIPY-derived dye designed for lipid droplet staining, cellular lipid imaging, and fluorescence microscopy. Its extended conjugated structure provides absorption near 627 nm and emission near 640 nm. Additionally, its lipophilic properties support fluorescent visualization of lipid-rich structures, making it useful for lipid metabolism research and cellular imaging.
Key Features and Benefits
- Red Fluorescent Lipid Stain: Exhibits typical absorption near 627 nm and emission near 640 nm, supporting red-channel fluorescence detection.
- BODIPY-Derived Fluorophore: Features a boron-dipyrromethene core with extended conjugation for red fluorescence emission.
- Extended Conjugated Structure: Contains two phenylethenyl substituents that shift the dye’s optical properties toward longer wavelengths.
- Lipid Droplet Staining: Supports fluorescent visualization of intracellular lipid droplets and other compatible lipid-rich structures.
- Lipophilic Dye Properties: Promotes association with hydrophobic cellular environments for lipid-focused fluorescence analysis.
- Fluorescence Microscopy Compatibility: Enables cellular imaging using microscopes equipped with appropriate red fluorescence excitation and emission filters.
- Cellular Lipid Visualization: Specifically supports investigation of lipid droplet distribution, accumulation, and intracellular localization.
- Spectral Separation: Red fluorescence facilitates combination with suitable green or blue fluorescent markers in multicolor imaging experiments.
- Research Versatility: Additionally, supports studies of lipid metabolism, intracellular lipid organization, and fluorescence-based cellular analysis.
Applications
- Lipid Droplet Imaging: Enables fluorescent visualization of lipid droplets in appropriately prepared biological samples.
- Cellular Lipid Staining: Supports detection of intracellular lipid-rich structures using compatible staining protocols.
- Fluorescence Microscopy: Facilitates imaging of lipid-associated fluorescence using suitable red-channel detection systems.
- Lipid Metabolism Research: Supports investigation of cellular lipid accumulation, storage, and distribution.
- Intracellular Lipid Localization: Specifically enables visualization of lipid droplet organization and distribution within cells.
- Multicolor Fluorescence Imaging: Supports combination with spectrally compatible fluorescent probes for cellular imaging studies.
- Lipid Storage Studies: Facilitates investigation of changes in cellular lipid droplets under different experimental conditions.
- Cell Biology Research: Provides a fluorescent probe for studying lipid-rich cellular compartments and related biological processes.
- Fluorescence-Based Assay Development: Additionally, supports development of imaging assays for lipid droplet visualization and cellular lipid analysis.
Storage and Handling
- Storage: Store at −20°C in a tightly sealed container, protected from light and moisture, unless product-specific documentation specifies otherwise. Additionally, minimize temperature fluctuations to preserve fluorescence stability.
- Handling: Use clean, dry equipment and appropriate protective gear. Prepare stock solutions in a compatible organic solvent, such as DMSO, after verifying solubility.
- Lipid Staining: Dilute the stock solution in a suitable staining medium and incubate with prepared samples. Subsequently, optimize dye concentration, incubation time, and washing conditions to minimize background fluorescence.
- Fluorescence Analysis: Detect fluorescence using appropriate excitation and emission settings near 627/640 nm. Evaluate lipid droplet localization and staining intensity using fluorescence microscopy or suitable imaging software.
(E,E)-3,5-Bis(phenylethenyl)-BDP Lipid Stain combines a red fluorescent BODIPY-derived structure with lipophilic properties for cellular lipid visualization. Its extended conjugation supports red fluorescence detection, while its lipid affinity facilitates imaging of intracellular lipid droplets. Therefore, it provides a useful research dye for lipid metabolism studies, fluorescence microscopy, and cellular lipid imaging.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
