AFDye 488 Alkyne

AFDye 488 Alkyne (CAS: 1006592-47-7)

AFDye 488 Alkyne is a green-fluorescent dye, equivalent to Alexa Fluor® 488, featuring an alkyne group for click chemistry. It reacts with azide-containing molecules through copper-catalyzed azide-alkyne cycloaddition (CuAAC), forming stable triazole linkages. This dye is ideal for imaging low-abundance azide-tagged biomolecules.

Key Features:

• Bright Fluorescence: Excites at 493 nm and emits at 518 nm, providing strong green signals for imaging.
• Click Chemistry Compatibility: The alkyne group enables efficient conjugation with azide-tagged molecules.
• High Photostability: Maintains fluorescence over time, making it suitable for extended imaging.

Applications:

• Imaging: Detects low-abundance azide-tagged biomolecules in fluorescence microscopy.
• Flow Cytometry: Labels cells for analysis with bright and stable fluorescence.
• Bioconjugation: Attaches to azide-modified biomolecules for research applications.

Handling and Storage:

• Storage: Store at -20°C, away from light and moisture.
• Handling: Follow standard laboratory precautions when using this reagent.