This product provides a bifunctional linker that combines a dibenzocyclooctyne (DBCO) group, C3 spacer, and primary amine. Its DBCO group enables copper-free click chemistry with azides, while the amine supports coupling with compatible amine-reactive molecules.
Key Features and Benefits
- Bifunctional Linker: DBCO-C3-Amine combines click-reactive DBCO and primary amine functionality within one molecule.
- DBCO Functionality: The strained cyclooctyne group reacts selectively with azides through strain-promoted azide-alkyne cycloaddition (SPAAC).
- Copper-Free Click Chemistry: DBCO specifically enables azide conjugation without requiring a copper catalyst.
- C3 Spacer: The three-carbon spacer specifically provides compact separation between the DBCO group and primary amine.
- Primary Amine Functionality: The terminal amine specifically provides a reactive site for coupling with NHS esters and activated carboxyl groups.
- Stable Triazole Formation: SPAAC reactions between DBCO and azides form stable triazole linkages.
- Sequential Functionalization: The two different reactive groups support stepwise conjugation and linker modification strategies.
- Versatile Conjugation Platform: DBCO-C3-Amine supports bioconjugation, molecular labeling, surface modification, and material functionalization.
Applications
- SPAAC Click Chemistry: DBCO-C3-Amine reacts with azide-functionalized molecules through copper-free click chemistry.
- Bioconjugation: Supports conjugation of compatible peptides, proteins, polymers, probes, and other biomolecules.
- Amine Coupling: The primary amine reacts with NHS esters and other compatible amine-reactive groups.
- Linker Modification: Provides DBCO functionality specifically for incorporation into molecules containing amine-reactive groups.
- Molecular Labeling: Supports attachment of DBCO functionality to compatible dyes, probes, and labeling reagents.
- Polymer Functionalization: Enables introduction of DBCO groups into compatible functional polymers.
- Surface Modification: Supports click-reactive modification of appropriately functionalized surfaces and materials.
- Biomaterial Research: Provides a versatile linker for developing functional conjugates and modified biomaterials.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions.
- Handling: Protect from moisture, excessive heat, and prolonged light exposure. Use conditions appropriate for DBCO-containing research reagents.
This product combines DBCO click chemistry with primary amine reactivity in a compact bifunctional linker and specifically supports SPAAC, bioconjugation, amine coupling, molecular labeling, polymer functionalization, and surface modification.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
