APDye 488 C6 Maleimide combines a bright, water-soluble green fluorescent dye with a C6 spacer and thiol-reactive maleimide group. Designed for excitation with the 488 nm laser line, it specifically supports sensitive fluorescent labeling of proteins, oligonucleotides, ligands, and other thiol-containing molecules.
Key Features and Benefits
- Bright Green Fluorescence: Provides especially strong green fluorescence with high brightness for sensitive detection, imaging, and molecular tracking.
- High Quantum Yield: Offers a fluorescence quantum yield approaching 100%, supporting strong and consistent fluorescent signals.
- 488 nm Laser Compatibility: Well suited for instruments and fluorescence systems specifically using the commonly available 488 nm laser line.
- Broad pH Stability: Maintains relatively stable fluorescence across approximately pH 4–10, providing flexibility across different experimental conditions.
- Water-Soluble Fluorophore: Good aqueous compatibility supports labeling and fluorescence studies specifically involving biological molecules.
- C6 Spacer: A six-carbon spacer separates the fluorophore from the reactive maleimide group, providing additional flexibility during conjugation.
- Thiol-Selective Maleimide: Reacts efficiently with reduced thiol (–SH) groups, particularly under mildly neutral conditions around pH 6.5–7.5.
- Stable Thioether Formation: Maleimide-thiol coupling forms stable covalent thioether linkages with cysteine-containing and other thiol-functionalized molecules.
- High Photostability: Designed to resist photobleaching and maintain fluorescence during extended detection and imaging experiments.
- Fluorescent Dye Alternative: Designed as a high-performance alternative to commonly used 488 nm green fluorophores for research labeling applications.
Applications
- Protein Labeling: Selective fluorescent conjugation to proteins containing accessible cysteine or other reduced thiol groups.
- Oligonucleotide Labeling: Fluorescent modification of thiol-functionalized oligonucleotides for nucleic acid research and detection.
- Peptide Labeling: Thiol-selective labeling of cysteine-containing peptides and peptide conjugates.
- Ligand Conjugation: Fluorescent tagging of thiol-functionalized ligands and other small molecular probes.
- Bioconjugation: Covalent attachment to compatible thiol-containing biomolecules through maleimide chemistry.
- Fluorescence Imaging: Bright green fluorescence for visualization, localization, and tracking of labeled molecules.
- Molecular Tracking: Useful for studying the distribution, localization, and interactions of appropriately labeled biomolecules.
- Biomaterial Research: Fluorescent functionalization of compatible polymers, nanoparticles, surfaces, and other research materials.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions. Protect from prolonged light exposure.
- Handling: Protect from moisture, excessive heat, and prolonged light. Minimize prolonged aqueous exposure to reduce maleimide hydrolysis. For thiol conjugation, mildly neutral conditions around pH 6.5–7.5 generally support selective maleimide-thiol coupling.
APDye 488 C6 Maleimide combines bright green fluorescence, high photostability, water solubility, and selective thiol-reactive chemistry, while its 488 nm laser compatibility and C6 maleimide functionality make it useful for fluorescent labeling across biochemical and cell biology research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
