Fluorescein-PEG6-Azide combines a fluorescein fluorophore, hydrophilic PEG6 spacer, and terminal azide functionality in one fluorescent linker and specifically supports fluorescence labeling, click chemistry, bioconjugation, and molecular tracking research.
Key Features and Benefits
- Fluorescent Label: Fluorescein provides a widely used fluorescent signal for labeling, detection, and imaging applications.
- PEG6 Spacer: A six-unit PEG spacer provides hydrophilicity and spatial separation between the fluorophore and reactive azide group.
- Azide Functionality: Terminal azide (–N₃) specifically enables selective conjugation with compatible alkyne and cyclooctyne groups.
- CuAAC Click Chemistry: Reacts with terminal alkynes through copper-catalyzed azide-alkyne cycloaddition to form stable triazole linkages.
- SPAAC Click Chemistry: Compatible with strained cyclooctynes such as DBCO for copper-free click conjugation.
- Stable Conjugation: Click reactions produce stable covalent triazole linkages especially suitable for labeling and bioconjugation studies.
- Hydrophilic Linker: The PEG6 spacer can improve aqueous compatibility while reducing steric interference during conjugation.
- Dual Functionality: Combines fluorescent detection and click-reactive functionality within a single molecular linker.
Applications
- Fluorescence Labeling: Fluorescent labeling and visualization of compatible molecules, polymers, and biomaterials.
- Click Chemistry: CuAAC and SPAAC reactions with compatible alkyne or cyclooctyne-functionalized molecules.
- Bioconjugation: Fluorescent modification of peptides, proteins, polymers, and other appropriately functionalized molecules.
- Molecular Labeling: Incorporation of a fluorescein reporter into click-compatible molecular constructs.
- Polymer Functionalization: Fluorescent labeling and modification of azide- or alkyne-compatible polymer systems.
- Surface Modification: Click-mediated fluorescent functionalization of compatible surfaces and materials.
- Nanoparticle Research: Fluorescent labeling and tracking of appropriately functionalized nanoparticles and nanomaterials.
- Biomaterial Research: Development and characterization of fluorescent, click-functionalized biomaterial systems.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions. Protect from prolonged light exposure.
- Handling: Protect from moisture, excessive heat, and prolonged light. Allow the material to reach room temperature before opening to minimize condensation.
Fluorescein-PEG6-Azide provides fluorescent detection and azide-based click reactivity within a compact hydrophilic linker, while its PEG6 spacer supports flexible conjugation for labeling, imaging, and functional material research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
