APDye 488 Azide C5
This product is a bright, green-fluorescent dye containing an azide group and a C5 linker. It specifically enables copper-catalyzed or copper-free click chemistry for labeling biomolecules, probes, and imaging agents with high sensitivity.
Key Features and Benefits
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Azide Group: Reacts with alkynes via CuAAC or SPAAC click chemistry.
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C5 Linker: Provides flexibility and spacing for efficient conjugation.
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Bright Fluorescence: Emits strong green signal (Ex/Em ≈ 495/519 nm).
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High Sensitivity: Enables detection in imaging, flow cytometry, and assays.
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High Purity: Ensures reproducibility in biological and chemical labeling.
Applications
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Bioconjugation: Labels proteins, peptides, and nucleic acids via click chemistry.
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Probe Development: Builds fluorescent probes for diagnostics and imaging.
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Cell Imaging: Provides bright, photostable fluorescence in live or fixed cells.
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Assay Development: Useful in ELISA, FRET, and fluorescence-based detection.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light-protected container.
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Handling: Dissolve in DMSO or aqueous buffers; avoid repeated freeze–thaw cycles.
This product integrates azide click reactivity with bright green fluorescence, specifically providing a versatile dye for labeling, probes, and imaging.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
