APDye 488 TCO (5-Isomer) provides a green fluorescent dye functionalized with trans-cyclooctene (TCO) for bioorthogonal labeling, fluorescence imaging, and bioconjugation research and specifically combines an APDye 488 fluorophore with a reactive TCO group that undergoes rapid inverse-electron-demand Diels–Alder (IEDDA) cycloaddition with tetrazine-functionalized molecules. This 5-isomer derivative specifically supports catalyst-free conjugation of biomolecules, imaging probes, and functionalized research compounds.
Key Features and Benefits
- Green Fluorescent Dye: Features an APDye 488 fluorophore designed specifically for green fluorescence detection and molecular imaging applications.
- Reactive TCO Functionality: Contains a trans-cyclooctene group specifically for rapid conjugation with tetrazine-functionalized molecules through bioorthogonal click chemistry.
- 5-Isomer Structure: Provides a defined positional isomer for fluorescence labeling and chemical conjugation research.
- Copper-Free Click Chemistry: Supports catalyst-free tetrazine-TCO ligation without requiring copper catalysts or additional coupling reagents.
- Rapid Bioorthogonal Conjugation: Enables efficient IEDDA cycloaddition with compatible tetrazine derivatives under suitable reaction conditions.
- Fluorescence Detection Compatibility: Supports green-channel fluorescence analysis using compatible excitation sources and emission filters.
- Selective Molecular Labeling: Enables fluorescent labeling of tetrazine-functionalized biomolecules with minimal interference from common biological functional groups.
- Stable Covalent Conjugation: Forms covalent cycloaddition products specifically through tetrazine-TCO reactions for preparing fluorescent molecular constructs.
- Versatile Imaging Applications: Supports fluorescence microscopy, flow cytometry, and molecular probe development using compatible labeling strategies.
Applications
- Bioorthogonal Click Chemistry: Supports tetrazine-TCO ligation for fluorescent labeling of appropriately functionalized biomolecules and synthetic compounds.
- Fluorescence Microscopy: Enables visualization of tetrazine-labeled molecular targets using compatible green fluorescence imaging systems.
- Protein Fluorescent Labeling: Supports preparation of fluorescent protein conjugates containing suitably introduced tetrazine groups.
- Peptide Bioconjugation: Enables attachment of green fluorescent labels to tetrazine-functionalized peptides for molecular interaction studies.
- Cellular Imaging Research: Supports fluorescence-based visualization of labeled molecular targets in compatible cellular research systems.
- Flow Cytometry: Useful for investigating fluorescently labeled cells and biomolecules using suitable green fluorescence detection channels.
- Antibody Labeling Research: Enables preparation of fluorescent antibody conjugates through tetrazine-functionalized antibody intermediates.
- Molecular Probe Development: Supports synthesis of fluorescent probes for investigating molecular recognition, binding, and biological interactions.
- Nanoparticle Functionalization: Enables attachment of fluorescent dye functionality to tetrazine-modified nanoparticles, polymers, and biomaterials.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed, light-protected container unless otherwise specified. Minimize exposure to moisture, excessive heat, and prolonged light to preserve dye fluorescence and TCO reactivity.
- Handling: Protect solutions from light and prepare fresh working solutions when possible. Use compatible organic solvents, such as DMSO or DMF, according to the product’s solubility characteristics. For bioorthogonal conjugation, react the TCO-functionalized dye with suitable tetrazine-containing molecules under optimized conditions. Avoid prolonged storage in solution and conditions that promote TCO isomerization or degradation.
APDye 488 TCO (5-Isomer) combines green fluorescence with reactive trans-cyclooctene functionality for selective, catalyst-free bioconjugation and specifically supports tetrazine-TCO click chemistry, protein and peptide labeling, fluorescence microscopy, flow cytometry, and molecular imaging research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
