APDye Fluor 488 (AF488 or Alexa Fluor 488 equivalent) is a bright green, water-soluble fluorescent dye optimized for 488 nm lasers. It provides stable fluorescence across a wide pH range, making it perfect for imaging and flow cytometry. AF488 offers high labeling efficiency with minimal quenching, suitable for conjugating to antibodies, proteins, peptides, and other biomolecules. Available in various conjugates, AF488 is ideal for diverse bioconjugation applications, including click chemistry and thiol-reactive conjugations.
APDye Fluor 488
APDye Fluor 488 (AF488 or Alexa Fluor 488 equivalent) – Bright Green Fluorescent Dye
Overview: APDye Fluor 488 (AF488) is a water-soluble, green-fluorescent dye that’s perfect for use with a 488 nm laser. It delivers bright, stable fluorescence that is not affected by pH changes, making it ideal for imaging and flow cytometry applications.
Key Features:
- Fluorescence Properties:
- Color: Bright green
- Laser Line: Optimized for 488 nm
- pH Stability: Consistent fluorescence from pH 4 to pH 10
- High Labeling Efficiency: Achieve high levels of labeling with minimal quenching
- Versatility:
- Applications: Can be conjugated to antibodies, peptides, proteins, tracers, and amplification substrates
- Uses: Perfect for both imaging and flow cytometry
Available Conjugates:
AxisPharm provides a variety of APDye Fluor 488 conjugates, including:
- APDye-488-NHS: For amine coupling
- APDye-488-amine: Direct amine conjugation
- APDye-488-DBCO: For click chemistry applications
- APDye-488-BCN: Another option for click chemistry
- APDye-488-azide: For click chemistry and labeling
- APDye-488-maleimide: For thiol-reactive conjugations
Bioconjugation Kits:
We also offer APDye-488 bioconjugation kits to simplify the process of conjugating the dye with your target molecules.
Benefits:
- Bright and Stable: Provides strong, clear fluorescence for accurate results.
- pH Stable: Maintains performance across a wide pH range.
- Efficient Labeling: Allows for high levels of labeling with minimal signal loss.
Summary: APDye Fluor 488 (AF488) is an excellent choice for green fluorescence applications. It offers bright, stable fluorescence and is compatible with a range of conjugates and bioconjugation kits, making it ideal for imaging and flow cytometry.
Ref:
Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB. Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions. Anal Biochem. 2002 Sep 1;308(1):18-25.