APDye 488 is a fluorescent dye that is completely identical to Alexa Fluor™ 488. APDye 488 is a fluorophore pH-insensitive over a broad range from 4 to 10. It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows biological objects detection with high sensitivity and a long excited-state lifetime.
APDye 488 C3 azide interacts with alkynyl biomolecules derivatives in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.
AxisPharm offers 4000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.