BDP TMR is a full analog of BODIPY™, an orange fluorescent dye. Due to its small size and relatively long excitation lifetime, this fluorophore can be used to study ligand-receptor interactions based on fluorescence polarization.
BDP TMR
BDP TMR: Spectral Properties and Applications
BDP TMR (Boron-Dipyrromethene Tetramethylrhodamine) is a red-orange fluorescent dye known for its excellent photostability, high brightness, and distinct spectral characteristics. It is commonly used in various fluorescence-based assays, including microscopy, FRET, and flow cytometry, due to its sharp and reliable fluorescence signals.
Spectral Properties of BDP TMR:
- Absorption Maximum: ~544 nm
- Emission Maximum: ~570 nm
- Stokes Shift: ~26 nm
- High Quantum Yield: Offers bright and efficient fluorescence, making it suitable for sensitive detection.
- Photostability: BDP TMR maintains fluorescence over prolonged exposure, resisting photobleaching.
- Sharp Spectra: Narrow absorption and emission peaks reduce spectral overlap, enhancing clarity in multiplex applications.
Applications of BDP TMR:
- Fluorescence Microscopy: Ideal for imaging cellular components and tracking biological processes with high precision.
- Flow Cytometry: Provides clear and distinct signals for cell sorting and phenotyping.
- FRET Studies: Used in FRET assays to study molecular interactions and dynamics with high sensitivity.
- Molecular Probes: Effective for labeling proteins, nucleic acids, and other biomolecules in diverse research fields.
BDP TMR’s bright, stable fluorescence and well-defined spectral properties make it an excellent choice for advanced fluorescence imaging and analysis, providing reliable and precise results across a range of applications.