APDye Fluor 488
APDye Fluor 488 (AF488 or Alexa Fluor 488 equivalent) – Bright Green Fluorescent Dye
Overview: APDye Fluor 488 (AF488) is a water-soluble, green-fluorescent dye that’s perfect for use with a 488 nm laser. It delivers bright, stable fluorescence that is not affected by pH changes, making it ideal for imaging and flow cytometry applications.
Key Features:
- Fluorescence Properties:
- Color: Bright green
- Laser Line: Optimized for 488 nm
- pH Stability: Consistent fluorescence from pH 4 to pH 10
- High Labeling Efficiency: Achieve high levels of labeling with minimal quenching
- Versatility:
- Applications: Can be conjugated to antibodies, peptides, proteins, tracers, and amplification substrates
- Uses: Perfect for both imaging and flow cytometry
Available Conjugates:
AxisPharm provides a variety of APDye Fluor 488 conjugates, including:
- APDye-488-NHS: For amine coupling
- APDye-488-amine: Direct amine conjugation
- APDye-488-DBCO: For click chemistry applications
- APDye-488-BCN: Another option for click chemistry
- APDye-488-azide: For click chemistry and labeling
- APDye-488-maleimide: For thiol-reactive conjugations
Bioconjugation Kits:
We also offer APDye-488 bioconjugation kits to simplify the process of conjugating the dye with your target molecules.
Benefits:
- Bright and Stable: Provides strong, clear fluorescence for accurate results.
- pH Stable: Maintains performance across a wide pH range.
- Efficient Labeling: Allows for high levels of labeling with minimal signal loss.
Summary: APDye Fluor 488 (AF488) is an excellent choice for green fluorescence applications. It offers bright, stable fluorescence and is compatible with a range of conjugates and bioconjugation kits, making it ideal for imaging and flow cytometry.
Ref:
Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB. Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions. Anal Biochem. 2002 Sep 1;308(1):18-25.