Thiol-PEG5-Azide
Thiol-PEG5-azide is a high-purity heterobifunctional PEG linker with a thiol group for selective coupling to maleimides, haloacetyls, disulfides, or gold surfaces, and an azide for copper-catalyzed or strain-promoted alkyne–azide cycloaddition (CuAAC or SPAAC) click chemistry. The PEG5 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling especially efficient, orthogonal bioconjugation in aqueous and organic systems.
Key Features and Benefits
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Thiol Group – Reacts selectively with maleimides, haloacetyls, disulfides, and gold surfaces.
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Azide Group – Compatible with CuAAC and SPAAC click chemistry for bioorthogonal conjugation.
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PEG5 Spacer – Improves solubility, flexibility, and accessibility of reactive groups.
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Orthogonal Reactivity – Enables sequential, site-specific modifications without cross-reactivity.
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High Purity – Supplied at >95% purity for consistent, reproducible results.
Applications
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Dual-functional linker for orthogonal conjugation
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Protein, peptide, and small molecule modification
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Surface immobilization on gold substrates and nanoparticles
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Multifunctional probe and diagnostic agent synthesis
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Drug delivery and ADC linker strategies
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light
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Handling: Use anhydrous solvents such as DMSO or DMF; protect thiol from oxidation by working under inert atmosphere or using stabilizers
This product combines thiol-based coupling and azide click chemistry in one linker, with a PEG5 spacer for solubility and flexibility, specifically enabling efficient, orthogonal bioconjugation for research and therapeutic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
