Tetrazine-Diazo-PEG4-Biotin is a multifunctional reagent combining a tetrazine group for rapid, bioorthogonal IEDDA reactions, a diazo group for photoinduced covalent cross-linking, and a biotin moiety for strong, non-covalent binding to avidin/streptavidin systems. The PEG4 spacer improves solubility and reduces steric hindrance, making it a versatile tool for labeling, cross-linking, and purification in chemical biology and diagnostics.
Features
- Tetrazine Group: Enables fast and selective IEDDA reactions with strained alkenes (e.g., TCO).
- Diazo Group: Facilitates photoactivated covalent cross-linking.
- Biotin Moiety: Strong binding for purification and detection.
- PEG4 Spacer: Enhances solubility and reduces steric hindrance.
- High Purity: ≥95% for reliable experimental results.
Applications
- Labeling: Bioorthogonal conjugation of biomolecules for imaging or isolation.
- Cross-Linking: Photoinduced covalent bonding in complex systems.
- Purification: Target enrichment using avidin/streptavidin systems.
- Imaging: Development of fluorescent or radiolabeled probes.
- Diagnostics: Functionalization for biosensor development.
Technical Details
- Purity: ≥95%
- Solubility: Water, DMSO, and other polar solvents.
- Storage: Store at -20°C, protected from light and moisture.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
