TCO-PEG4-Amido Maleimide
This high-purity heterobifunctional PEG linker has a trans-cyclooctene (TCO) group for rapid, catalyst-free inverse electron-demand Diels–Alder (IEDDA) cycloaddition with tetrazines and a maleimide specifically for selective thiol coupling. The PEG4 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling efficient, orthogonal bioconjugation in aqueous and organic systems.
Key Features and Benefits
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TCO Group – Enables ultra-fast, catalyst-free tetrazine ligation for bioorthogonal labeling.
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Maleimide Group – Reacts selectively with thiols via Michael addition under mild conditions.
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PEG4 Spacer – Improves solubility and accessibility of reactive groups while reducing steric hindrance.
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Orthogonal Reactivity – Supports sequential, site-specific modifications without cross-reactivity.
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High Purity – Supplied at >95% purity for consistent, reproducible results.
Applications
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Dual-functional linker for orthogonal conjugation
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Protein, peptide, and antibody modification
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Antibody–drug conjugate (ADC) linker chemistry
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Multifunctional imaging and diagnostic probe synthesis
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light
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Handling: Use anhydrous solvents such as DMSO or DMF; avoid prolonged moisture exposure to preserve reactivity
Summary: TCO-PEG4-amido maleimide combines tetrazine ligation via TCO with thiol–maleimide conjugation, linked by a PEG4 spacer for solubility and flexibility, making it ideal for precise, orthogonal bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
