Copper Free Click Chemistry Linkers

Copper free click chemistry linkers have been widely used for in vivo bioconjugation applications. Conventional “Click Chemistry” requires the presence of cytotoxic of Cu(I) catalyst, which limits its applications in biological systems. The Copper-free Click Chemistry is based on the reaction of a cyclooctyne (DBCO and BCN) moiety with an azide-labeled reaction partner, known as strain-promoted alkyne azide cycloaddition (SPAAC).

This new “Click Chemistry” is very fast at room temperature and does not require a cytotoxic Cu(I) catalyst. As a result, the copper free click chemistry has been advanced in molecular imaging and drug delivery for diagnosis and therapy.

Cyclooctynes are thermostable and react specifically toward azides to stable triazoles form in quantitative yields. The copper free click chemistry promoted bioconjugation occurs when a DBCO activated biomolecule react with a azide modified biomolecule form a bioconjugate. The process provides a wide range of benefits, including :

1. Biocompatibility – no cytotoxic Copper catalyst required – ideal for in-vivo applications

2. Mild conditions – conjugation occurs in aqueous buffered media and at low temperature with ultrafast kinetics

3. Stability – DBCO, BCN and azide moieties are stable and suitable for long term storage

4. Efficiency – the formation of stable triazole is quantitative

5. Specific and Bioorthogonal – only azide reacts with DBCO and BCN specifically, various functional groups such as -NH2, -SH, -COOH and other protein functionalities don’t interfere.

AxisPharm is specialized in various copper free click chemistry tool development. We provide solution to custom synthesize and pegylate DBCO, TCO, BCN, cyclopropene, tetrazine linkers.

Did not find what you are looking for? Give us a call at 858-677-9432 or email us at Our scientific advisors are ready to help.

TCO Linkers
BCN Linkers
DBCO Linkers
Tetrazine Linkers

Catalog No. Name Structure M.W. Purity Pricing
AP10123 (2-methylcycloprop-2-en-1-yl)methanol (2-methylcycloprop-2-en-1-yl)methanol 84.12 95% Pricing


Unit Price(USD) Quantity
100mg $690.00
250mg $990.00
500mg $1,550.00
1g $1,990.00
AP10124 (2-methylcyclopropenyl)methyl 4-nitrophenyl carbonate (2-methylcyclopropenyl)methyl 4-nitrophenyl carbonate 249.22 95% Pricing


(2-methylcyclopropenyl)methyl 4-nitrophenyl carbonate
Unit Price(USD) Quantity
100mg $1,090.00
250mg $1,580.00
500mg $2,280.00
1g $2,880.00
AP11003 2-(2-aminoethyldisulfanyl)acetic acid 2-(2-aminoethyldisulfanyl)acetic acid 167.24 Pricing


2-(2-aminoethyldisulfanyl)acetic acid
Unit Price(USD) Quantity
100mg $640.00
250mg $1,200.00
500mg $1,680.00
AP11008 2-[(2,4-dinitrophenyl)disulfanyl]ethanol 2-[(2,4-dinitrophenyl)disulfanyl]ethanol 276.28 Pricing


Unit Price(USD) Quantity
100mg $320.00
250mg $490.00
500mg $780.00
AP11002 2-Boc-aminoethyl 2'-hydroxyethyl disulfide 2-Boc-aminoethyl 2'-hydroxyethyl disulfide 253.38 Pricing


2-Boc-aminoethyl 2'-hydroxyethyl disulfide
Unit Price(USD) Quantity
100mg $320.00
250mg $490.00
500mg $780.00
AP10127 2-methyl-3-(trimethylsilyl)cycloprop-2-ene-1-carboxylic acid 2-methyl-3-(trimethylsilyl)cycloprop-2-ene-1-carboxylic acid 170.28 95% Pricing


2-methyl-3-(trimethylsilyl)cycloprop-2-ene-1-carboxylic acid
Unit Price(USD) Quantity
100mg $920.00
250mg $1,200.00
500mg $1,800.00
1g $2,700.00
AP10693 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol 188.2 95% Pricing


Unit Price(USD) Quantity
250mg $380.00
500mg $580.00
1g $890.00
AP10999 Acetic acid, 2,2'-dithiobis-dihydrazide Acetic acid, 2,2'-dithiobis-dihydrazide 210.27 Pricing


Acetic acid, 2,2'-dithiobis-dihydrazide
Unit Price(USD) Quantity
500mg $880.00
100mg $340.00
250mg $520.00
AP10143 ald-benzyl-amine-PEG2-TCO ald-benzyl-amine-PEG2-TCO 446.54 95% Pricing


Unit Price(USD) Quantity
100mg $750.00
250mg $1,500.00
500mg $2,250.00
1g $3,380.00
AP11007 Amino-ethyl-SS-PEG3-NHBoc Amino-ethyl-SS-PEG3-NHBoc 384.55 Pricing


Unit Price(USD) Quantity
100mg $280.00
250mg $430.00
500mg $680.00