Copper Free Click Chemistry Linkers

Copper free click chemistry linkers have been widely used for in vivo bioconjugation applications. Conventional “Click Chemistry” requires the presence of cytotoxic of Cu(I) catalyst, which limits its applications in biological systems. The Copper-free Click Chemistry is based on the reaction of a cyclooctyne (DBCO and BCN) moiety with an azide-labeled reaction partner, known as strain-promoted alkyne azide cycloaddition (SPAAC).

This new “Click Chemistry” is very fast at room temperature and does not require a cytotoxic Cu(I) catalyst. As a result, the copper free click chemistry has been advanced in molecular imaging and drug delivery for diagnosis and therapy.

Cyclooctynes are thermostable and react specifically toward azides to stable triazoles form in quantitative yields. The copper free click chemistry promoted bioconjugation occurs when a DBCO activated biomolecule react with a azide modified biomolecule form a bioconjugate. The process provides a wide range of benefits, including :

1. Biocompatibility – no cytotoxic Copper catalyst required – ideal for in-vivo applications

2. Mild conditions – conjugation occurs in aqueous buffered media and at low temperature with ultrafast kinetics

3. Stability – DBCO, BCN and azide moieties are stable and suitable for long term storage

4. Efficiency – the formation of stable triazole is quantitative

5. Specific and Bioorthogonal – only azide reacts with DBCO and BCN specifically, various functional groups such as -NH2, -SH, -COOH and other protein functionalities don’t interfere.

AxisPharm is specialized in various copper free click chemistry tool development. We provide solution to custom synthesize and pegylate DBCO, TCO, BCN, cyclopropene, tetrazine linkers.

Did not find what you are looking for? Give us a call at 858-677-9432 or email us at Our scientific advisors are ready to help.

TCO Linkers BCN Linkers DBCO Linkers Tetrazine Linkers
Catalog No. Name Structure M.W. Purity Pricing
AP10123 (2-methylcycloprop-2-en-1-yl)methanol (2-methylcycloprop-2-en-1-yl)methanol 84.12 95% Pricing


Unit Price(USD) Quantity
100mg $690.00
250mg $990.00
500mg $1,550.00
1g $1,990.00
AP10124 (2-methylcyclopropenyl)methyl 4-nitrophenyl carbonate (2-methylcyclopropenyl)methyl 4-nitrophenyl carbonate 249.22 95% Pricing


(2-methylcyclopropenyl)methyl 4-nitrophenyl carbonate
Unit Price(USD) Quantity
100mg $790.00
250mg $1,090.00
500mg $1,650.00
1g $2,090.00
AP10127 2-methyl-3-(trimethylsilyl)cycloprop-2-ene-1-carboxylic acid 2-methyl-3-(trimethylsilyl)cycloprop-2-ene-1-carboxylic acid 170.28 95% Pricing


2-methyl-3-(trimethylsilyl)cycloprop-2-ene-1-carboxylic acid
Unit Price(USD) Quantity
100mg $920.00
250mg $1,200.00
500mg $1,800.00
1g $2,700.00
AP10693 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol 188.2 95% Pricing


Unit Price(USD) Quantity
250mg $380.00
500mg $580.00
1g $890.00
AP10143 ald-benzyl-amine-PEG2-TCO ald-benzyl-amine-PEG2-TCO 446.54 95% Pricing


Unit Price(USD) Quantity
100mg $750.00
250mg $1,500.00
500mg $2,250.00
1g $3,380.00
AP10139 aminooxy-PEG2-BCN aminooxy-PEG2-BCN 397.47 95% Pricing


Unit Price(USD) Quantity
100mg $700.00
250mg $1,400.00
500mg $2,100.00
1g $3,150.00
AP10141 aminooxy-PEG2-TCO aminooxy-PEG2-TCO 387.48 95% Pricing


Unit Price(USD) Quantity
100mg $750.00
250mg $1,500.00
500mg $2,250.00
1g $3,380.00
AP10970 BCN-carbamate-PEG2-Bromide BCN-carbamate-PEG2-Bromide 388.3 95% Pricing


Unit Price(USD) Quantity
50 mg $520.00
100 mg $980.00
250 mg $1,980.00
AP10739 BCN-endo-PEG3-Maleimide BCN-endo-PEG3-Maleimide 519.6 95% Pricing


Unit Price(USD) Quantity
50mg $680.00
100mg $980.00
250mg $1,680.00
AP10738 BCN-exo-PEG7-Maleimide BCN-exo-PEG7-Maleimide 739.86 95% Pricing


Unit Price(USD) Quantity
50mg $680.00
100mg $980.00
250mg $1,680.00