TCO-PEG1-Amido Maleimide
This high-purity PEG linker features a TCO for rapid, catalyst-free IEDDA cycloaddition with tetrazines and a maleimide for selective thiol coupling. Its short PEG1 spacer specifically enhances solubility, reduces steric hindrance, and maintains a compact structure for efficient, orthogonal bioconjugation.
Key Features and Benefits
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TCO Group – Enables ultra-fast, catalyst-free tetrazine ligation for bioorthogonal labeling.
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Maleimide Group – Reacts selectively with sulfhydryl groups via Michael addition under mild conditions.
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PEG1 Spacer – Improves solubility and reduces steric hindrance while keeping linker length minimal.
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Orthogonal Reactivity – Allows sequential modifications without cross-reactivity.
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High Purity – Supplied at >95% purity for reproducible performance.
Applications
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Dual-functional linker for orthogonal conjugation
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Protein, peptide, and antibody modification
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Antibody–drug conjugate (ADC) linker design
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Multifunctional imaging and diagnostic probe development
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light
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Handling: Use anhydrous solvents such as DMSO or DMF; avoid prolonged moisture exposure to preserve reactivity
This product combines tetrazine ligation via TCO with thiol–maleimide conjugation, connected by a PEG1 spacer for solubility and minimal steric hindrance, making it especially ideal for precise, orthogonal bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
