α‑Hydroxyl‑ω‑biotin‑poly(D,L‑lactide) (PDLLA‑Biotin, MW 5K) is linear, mono‑biotin‑terminated fully amorphous biodegradable aliphatic polyester. D‑lactide and L‑lactide repeat units are randomly distributed along polymer backbone, disrupting stereoregularity and eliminating crystalline domains. Compared with lower‑molecular‑weight PDLLA‑Biotin analogues, this 5 kDa variant exhibits higher hydrophobicity and slower but homogeneous hydrolytic degradation. One chain terminus carries reactive biotin (vitamin H), the opposite end retains inert hydroxyl (‑OH). Terminal biotin enables high‑affinity specific binding with avidin / streptavidin, widely used for biomolecule immobilization, ligand capture and targeted bioconjugation. It serves as a monofunctional macro‑monomer for nanoparticle surface modification, biosensing and targeted drug‑delivery biomedical research.
Key Features
- MW: 5000 Da, linear α‑hydroxyl‑ω‑biotin‑terminated poly(D,L‑lactide)
- Fully amorphous non‑crystalline structure, random D/L‑lactide sequence, no melting point
- Higher hydrophobicity, slower yet uniform hydrolytic degradation versus low‑MW PDLLA‑Biotin
- Excellent biocompatibility; degradation produces non‑toxic lactic‑acid metabolites
- Single reactive biotin terminus; high‑affinity binding to avidin/streptavidin; opposite end is inert hydroxyl ‑OH
- Narrow molecular‑weight distribution, stable batch‑to‑batch reproducibility
- Soluble in DCM, CHCl₃, THF, DMF, acetone; insoluble in water
- Biotin moiety is light‑sensitive; avoid prolonged light exposure and high‑temperature conditions
Applications
- Monofunctional building block for avidin‑biotin mediated bioconjugation of peptides, antibodies, targeting ligands and fluorescent probes
- Biotin‑functional surface modification of PDLLA‑based nanoparticles and microspheres for controlled‑release study
- Precursor for biosensor construction and biomolecule immobilization onto biodegradable polyester substrates
- Research‑grade raw material for preparing avidin‑mediated targeted drug‑delivery carriers
Handling & Storage
- Store at −20 °C under dry inert nitrogen or argon atmosphere; strictly moisture‑proof, protected from light
- Keep hermetically sealed; moisture and heat may trigger ester hydrolysis and impair biotin activity; avoid repeated freeze‑thaw cycles
- For research‑only use; not intended for clinical or diagnostic applications
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.