PC Biotin-PEG3-NHS carbonate ester
PC Biotin-PEG3-NHS carbonate ester is a high-purity heterobifunctional linker with a photocleavable (PC) biotin moiety and an NHS carbonate ester for efficient amine coupling. The PEG3 spacer improves solubility, flexibility, and reduces steric hindrance. The NHS carbonate reacts with primary amines to form stable carbamate linkages, while the photocleavable group enables controlled biotin release upon light exposure, making it especially ideal for reversible labeling, affinity purification, and light-triggered release applications.
Key Features and Benefits
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Photocleavable Biotin: Allows controlled release from streptavidin/avidin complexes upon UV or visible light exposure.
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NHS Carbonate Ester: Efficiently couples with primary amines to form stable carbamates.
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PEG3 Spacer: Enhances solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Enables site-specific and reversible biotinylation.
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High Purity: Supplied at >95% purity for reproducible results.
Applications
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Light-controlled release in affinity purification workflows.
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Reversible biotinylation of proteins, peptides, and ligands.
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Photocleavable probes specifically for imaging and biosensing.
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Controlled drug delivery and release systems.
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Surface functionalization for photo-responsive biomaterials.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS carbonate reactivity.
This product combines a photocleavable biotin with an NHS carbonate ester for amine coupling, linked by a PEG3 spacer specifically for solubility and flexibility in reversible biotinylation and light-triggered release applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
