Cleavable biotin is a type of molecule that contains a biotin moiety and a cleavable linker. It is commonly used in drug research and development as a tool to selectively label and isolate target proteins for further characterization. The cleavable linker allows for the specific release of the labeled protein, providing a powerful method for the identification and quantification of protein-protein interactions and other molecular interactions.
Functional groups play a critical role in the design and synthesis of cleavable biotin molecules. The biotin moiety contains a carboxyl group, which can form hydrogen bonds with proteins and other biomolecules. Meanwhile, the cleavable linker typically contains reactive groups, such as disulfide or ester bonds, which can be selectively cleaved under specific conditions.
Cleavable biotin has gained significant attention in drug research and development due to its versatility and specificity. It has been used in a wide range of applications, including proteomics, drug discovery, and diagnostics. Its ability to selectively label and isolate target proteins has led to a better understanding of disease mechanisms and the identification of potential drug targets.