NO2A-Azide provides an azide-functionalized macrocyclic chelator for click chemistry, metal coordination, and molecular imaging research and specifically combines a 1,4,7-triazacyclononane-based NO2A framework with two acetic acid groups and an azide functionality. This bifunctional structure specifically supports conjugation with alkyne-containing molecules and preparation of metal-chelate constructs for radiopharmaceutical research.
Key Features and Benefits
- NO2A Macrocyclic Framework: Features a triazacyclononane-based chelator with two pendant acetate groups for coordination of compatible metal ions.
- Azide Functionality: Provides a reactive azide group for covalent attachment to alkyne-functionalized molecules through suitable click chemistry reactions.
- Click Chemistry Compatibility: Supports copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) for preparing stable triazole-linked molecular conjugates.
- Bifunctional Chelator Design: Combines metal coordination and chemical conjugation capabilities within a single functionalized macrocyclic compound.
- Bioorthogonal Conjugation Potential: Supports strain-promoted azide-alkyne cycloaddition (SPAAC) with compatible strained alkynes, such as DBCO derivatives.
- Radiometal Coordination: Supports investigation of complexation with compatible metal ions, including copper-64, under optimized reaction conditions.
- Stable Triazole Linkage: Enables formation of covalent triazole bonds through reactions with suitable terminal or strained alkynes.
- Versatile Biomolecular Modification: Supports attachment of NO2A functionality to alkyne-modified peptides, proteins, polymers, and targeting ligands.
Applications
- Click Chemistry Bioconjugation: Supports CuAAC-mediated attachment of NO2A chelators to alkyne-containing biomolecules and synthetic compounds.
- Copper-Free Click Chemistry: Enables SPAAC conjugation with strained alkyne-functionalized molecules without requiring copper catalysts.
- Radiopharmaceutical Research: Supports preparation of functionalized macrocyclic chelators for investigational radiometal labeling applications.
- Copper-64 Radiolabeling: Useful for investigating copper-64 coordination, radiochemical yield, and stability of NO2A-based complexes.
- Peptide Functionalization: Supports preparation of NO2A-modified peptides through conjugation with alkyne-containing peptide sequences.
- Protein Bioconjugation: Enables introduction of macrocyclic chelating groups into proteins containing appropriately incorporated alkyne functionalities.
- PET Imaging Research: Supports development of investigational radiometal-labeled probes for positron emission tomography.
- Polymer Modification: Useful for incorporating NO2A chelators into alkyne-functionalized polymers, nanoparticles, and biomaterials.
- Targeted Molecular Imaging: Supports preparation of chelator-functionalized targeting ligands for receptor-binding and imaging studies.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container unless otherwise specified. Protect from moisture, excessive heat, and prolonged light exposure.
- Handling: Handle using appropriate laboratory precautions and minimize trace-metal contamination. For CuAAC reactions, use suitable copper catalysts, ligands, and alkyne-functionalized reaction partners. For copper-free conjugation, use compatible strained alkynes such as DBCO. Remove residual copper when necessary before subsequent radiometal labeling or biological studies.
NO2A-Azide combines a macrocyclic NO2A chelator with an azide functionality for versatile click chemistry conjugation and specifically supports radiometal coordination, peptide and protein modification, molecular imaging, and development of functionalized radiopharmaceutical research compounds.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
