DOTAM-maleimide provides a bifunctional macrocyclic chelator for thiol-selective bioconjugation, metal coordination, and molecular imaging research and specifically combines the DOTAM (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetamide) chelating framework with a reactive maleimide group. This structure specifically supports covalent attachment to thiol-containing biomolecules while retaining the macrocyclic framework for metal complexation.
Key Features and Benefits
- DOTAM Macrocyclic Framework: Contains a tetraazacyclododecane-based chelator with four acetamide groups for coordination of compatible metal ions.
- Maleimide Functionality: Provides a thiol-reactive group for selective conjugation with cysteine residues and other sulfhydryl-containing molecules.
- Bifunctional Chelator Design: Combines metal coordination and covalent bioconjugation capabilities within a single functionalized molecule.
- Thiol-Selective Conjugation: Supports formation of stable thioether-linked conjugates through reactions with accessible sulfhydryl groups under suitable conditions.
- Lanthanide Coordination Capability: Enables investigation of DOTAM complexes with compatible lanthanide ions for coordination chemistry and imaging research.
- Site-Directed Protein Modification: Supports conjugation to accessible cysteine residues for controlled introduction of macrocyclic chelating groups.
- Mild Reaction Conditions: Maleimide-thiol coupling can proceed under relatively mild aqueous conditions compatible with many biomolecules.
- Versatile Functionalization: Enables attachment of DOTAM functionality to peptides, proteins, antibodies, and thiol-functionalized polymers.
Applications
- Antibody Bioconjugation: Supports attachment of DOTAM chelators to antibodies containing accessible or engineered cysteine residues.
- Peptide Modification: Enables preparation of DOTAM-functionalized peptides through maleimide coupling with cysteine-containing sequences.
- Protein Labeling Research: Useful for introducing metal-binding macrocycles into proteins through thiol-selective conjugation.
- Lanthanide Coordination Studies: Supports investigation of DOTAM-metal complex formation, coordination properties, and stability.
- Molecular Imaging Research: Enables development of metal-chelate biomolecular constructs for investigational imaging applications.
- MRI Contrast Agent Research: Supports exploration of DOTAM-based metal complexes and their magnetic resonance properties.
- Thiol-Functionalized Polymer Modification: Useful for attaching DOTAM groups to polymers and biomaterials containing reactive sulfhydryl groups.
- Targeted Probe Development: Supports preparation of chelator-functionalized targeting molecules for molecular recognition and imaging studies.
- Bioconjugation Method Development: Enables optimization of maleimide-thiol coupling efficiency, conjugate stability, and purification procedures.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container unless otherwise specified. Protect from moisture, light, and excessive heat.
- Handling: Minimize moisture exposure and prepare solutions shortly before use. Perform maleimide-thiol conjugation under suitable mildly acidic to neutral conditions, typically around pH 6.5–7.5. Avoid competing thiol-containing reagents during coupling. Minimize trace-metal contamination when preparing metal complexes.
DOTAM-maleimide combines a macrocyclic DOTAM chelator with thiol-reactive maleimide functionality for selective biomolecular modification and specifically supports antibody and peptide conjugation, lanthanide coordination, molecular imaging research, and development of functionalized metal-chelate systems.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
