NHS-C5-tris-PEG3-BCN (exo) combines an amine-reactive NHS ester, C5 linker, tris-PEG3 spacer, and exo-BCN functionality in a multifunctional conjugation reagent and specifically supports sequential bioconjugation, amine labeling, and copper-free SPAAC click chemistry with azide-containing molecules.
Key Features and Benefits
- Bifunctional Conjugation Reagent: Combines NHS ester and BCN functionalities specifically for sequential molecular modification and conjugation.
- NHS Ester Functionality: Reacts with primary amines on proteins, peptides, and other amine-containing molecules.
- C5 Linker: Provides additional spacing between the reactive NHS ester and the central linker architecture.
- Tris-PEG3 Spacer: Provides a flexible, hydrophilic PEG-containing framework that improves spacing between functional groups.
- Exo-BCN Functionality: The exo bicyclononyne group provides a strained alkyne for efficient reaction with azides.
- SPAAC Click Chemistry: BCN reacts with azide-functionalized molecules specifically through strain-promoted azide-alkyne cycloaddition.
- Copper-Free Conjugation: Enables azide-BCN coupling without a copper catalyst, specifically supporting bioorthogonal labeling strategies.
- Sequential Functionalization: NHS ester chemistry can introduce the BCN-containing linker onto amine-bearing molecules for subsequent azide conjugation.
Applications
- Bioconjugation: Supports sequential attachment of compatible molecules through amine coupling and SPAAC click chemistry.
- Amine Labeling: NHS ester functionality enables modification of primary amines on suitable biomolecules and synthetic compounds.
- SPAAC Click Chemistry: Exo-BCN supports copper-free conjugation with azide-functionalized reaction partners.
- Protein Modification: Useful for introducing BCN functionality onto proteins containing accessible primary amines.
- Peptide Modification: Supports amine-directed functionalization of peptides for subsequent click conjugation.
- Molecular Labeling: Enables attachment of compatible probes, labels, ligands, and other azide-functionalized molecules.
- Nanoparticle Functionalization: Supports modification and conjugation of appropriately functionalized nanoparticle systems.
- Biomaterial Research: Useful for constructing functional materials through amine-reactive and bioorthogonal conjugation strategies.
Storage and Handling
- Storage: Store according to the product specification in a tightly sealed container under dry conditions.
- Handling: NHS esters are moisture sensitive and can hydrolyze in aqueous environments. Minimize moisture exposure and prepare aqueous solutions shortly before use.
NHS-C5-tris-PEG3-BCN (exo) combines amine-reactive NHS chemistry with copper-free BCN click functionality, while its PEG-containing linker architecture supports sequential bioconjugation, molecular labeling, and functional material research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
